[二乙基亚硝胺诱导的大鼠肝癌中转化生长因子β表达与其附近肥大细胞的相关性研究]
[Correlation study of TGF beta expression in diethylnitrosamine-induced rat liver cancer and mast cells in its vicinity].
作者信息
Zheng M, Ruan Y, Wu Z
机构信息
Department of Pathology, Jiujiang Medical College, Jiujiang 332000, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2000 Nov;22(6):463-5.
OBJECTIVE
To study the correlation between tumor cell expression of TGF beta and the amount of surrounding mast cells in hepatocarcinogenesis.
METHODS
Hepatocarcinoma was induced by diethylnitrosamine (DENA) in rats. The expression of TGF beta was examined immunohistochemically, and mast cells were identified by Alcian blue staining. The results were undergone cell imaging analysis.
RESULTS
Positive expression of TGF beta was observed in the cytoplasm of the hepatocarcinoma cells. The intensity of expression differd in various groups of experiment. There was a positive correlation between the intensity of the TGF beta expression in hepatocarcinoma cells and the amount of mast cells surrounding the tumor nodules (P < 0.01).
CONCLUSION
Mast cell infiltration in DENA-induced rat hepatoma may be due to the chemotactic effect of TGF beta expressed in tumor cells.
目的
研究肝癌发生过程中肿瘤细胞转化生长因子β（TGFβ）的表达与周围肥大细胞数量之间的相关性。
方法
用二乙基亚硝胺（DENA）诱导大鼠发生肝癌。采用免疫组织化学法检测TGFβ的表达，并用阿尔辛蓝染色鉴定肥大细胞。对结果进行细胞成像分析。
结果
在肝癌细胞的细胞质中观察到TGFβ的阳性表达。不同实验组的表达强度有所不同。肝癌细胞中TGFβ表达强度与肿瘤结节周围肥大细胞数量呈正相关（P < 0.01）。
结论
DENA诱导的大鼠肝癌中肥大细胞浸润可能是由于肿瘤细胞中表达的TGFβ的趋化作用。
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