Kitazima K, Shibata S
J Lab Clin Med. 1975 May;85(5):855-64.
A coil planet centrifuge (CPC) is an apparatus aiming to obtain a centrifugal force exerting on particles suspended in a fluid in a coiled polyethylene tube (coil) which makes self-rotation around its axis and revolution around the principal axis simultaneously. If a linear osmotic gradient is prepared in the coil and a small amount of blood is applied to it and subjected to centrifugation, one is able to observe the membrane properties of erythrocytes in terms of the shift of hemolysis band appearing in the coil with high accuracy and precision. Coil planet centrifugation was applied to the observation of altered membrane properties of erythrocytes in hepatobiliary diseases. Remarkable shifts of end-point of the hemolysis band going in parallel with the severity of damage were visualized. Study as to its cause revealed that increased free cholesterol content of erythrocyte membrane which was the result of plasma cholesterol Esterstürz due to inhibition of lecithin-cholesterol acyltransferase by stagnant bile acids was responsible for this phenomenon. Elevation of serum bile acid concentration and increased serum leucine-aminopeptidase were closely correlated to the shift of hemolysis end-point toward the low osmolal side. CPC may be useful for detection and chasing the clinical course of patients with hepatobiliary disorders.
螺旋行星离心机（CPC）是一种旨在获得离心力的装置，该离心力作用于盘绕在聚乙烯管（螺旋管）中的流体中悬浮的颗粒上，螺旋管围绕其自身轴线自转并同时围绕主轴公转。如果在螺旋管中制备线性渗透梯度，并向其中加入少量血液并进行离心处理，就能够高精度、高精准度地根据螺旋管中出现的溶血带的移动来观察红细胞的膜特性。螺旋行星离心法被用于观察肝胆疾病中红细胞膜特性的改变。观察到溶血带终点的显著移动与损伤的严重程度平行。对其原因的研究表明，红细胞膜游离胆固醇含量增加是导致这种现象的原因，而这是由于胆汁酸停滞抑制卵磷脂胆固醇酰基转移酶导致血浆胆固醇酯水解的结果。血清胆汁酸浓度升高和血清亮氨酸氨基肽酶增加与溶血终点向低渗侧的移动密切相关。CPC可能有助于检测和追踪肝胆疾病患者的临床病程。
