Kim I S, Jeong Y I, Kim D H, Lee Y H, Kim S H
College of Pharmacy, Chosun University, Kwangju, Korea.
Arch Pharm Res. 2001 Feb;24(1):69-73. doi: 10.1007/BF02976496.
Biodegradable hydrogels based on glycidyl methacrylate dextran (GMD) and dimethacrylate poly(ethylene glycol) (DMP) were proposed for colon-specific drug delivery. GMD was synthesized by coupling of glycidyl methacrylate with dextran in the presence of 4-(N,N-dimethyl-amino)pyridine (DMAP) using dimethylsulfoxide as a solvent. Methacrylate-terminated poly (ethylene glycol) (PEG) macromer was prepared by the reaction of PEG with methacryloyl chloride. GMD/DMP hydrogels were prepared by radical polymerization of phosphate buffer solution (0.1M, pH 7.4) of GMD and DMP, using ammonium peroxydisulfate (APS) and UV as initiating system. The synthetic GMD, DMP, and GMD/DMP hydrogels were characterized by fourier transform infrared (FT-IR) spectroscopy. The FITC-albumin loaded hydrogels were prepared by adding FITC-albumin solution before UV irradiation. Swelling capacity of GMD/DMP hydrogels was controlled not only by molecular weight of dextran, but also by incorporation ratio of DMP Degradation of the hydrogels has been studied in vitro with dextranase. FITC-albumin release from the GMD/DMP hydrogels was affected by molecular weight of dextran and the presence of dextranase in the release medium.
基于甲基丙烯酸缩水甘油酯葡聚糖（GMD）和二甲基丙烯酸聚乙二醇（DMP）的可生物降解水凝胶被用于结肠特异性药物递送。GMD是在4-(N,N-二甲基氨基)吡啶（DMAP）存在下，以二甲基亚砜为溶剂，通过甲基丙烯酸缩水甘油酯与葡聚糖偶联合成的。甲基丙烯酸酯封端的聚乙二醇（PEG）大分子单体是通过PEG与甲基丙烯酰氯反应制备的。GMD/DMP水凝胶是通过GMD和DMP的磷酸盐缓冲溶液（0.1M，pH 7.4）在过硫酸铵（APS）和紫外线作为引发体系下进行自由基聚合制备的。合成的GMD、DMP和GMD/DMP水凝胶通过傅里叶变换红外（FT-IR）光谱进行表征。负载FITC-白蛋白的水凝胶是在紫外线照射前加入FITC-白蛋白溶液制备的。GMD/DMP水凝胶的溶胀能力不仅受葡聚糖分子量的控制，还受DMP掺入率的控制。已用葡聚糖酶在体外研究了水凝胶的降解。FITC-白蛋白从GMD/DMP水凝胶中的释放受葡聚糖分子量和释放介质中葡聚糖酶的存在影响。
