Gavrilov V B
Institute of Photobiology, Belarussian Academy of Sciences, ul. Akademicheskaya 27, Minsk, 220072 Belarus.
Biofizika. 2001 Jan-Feb;46(1):39-42.
The constant of binding of a new fluorescent probe pyrrone red to human serum albumin (Kb = 4.7.10(5) M-1) and the number of binding sites (N = 2) were determined by the method of double fluorimetric titration at a Fex/Fem intensity ratio of 560/625 nm. The affinity of pyrrone red for albumin was by 20% lower than that of 8-anilinonaphthalenesulfonate and 2.4 times higher than that of another probe K35. Thus, pyrrone red is almost identical to amlinonaphthalenesulfonate in affinity for albumin and can be considered as a long-wavelength "red" analogue of anilinonaphthalenesulfonate.
通过双荧光滴定法，在激发光强度比为560/625 nm的条件下，测定了新型荧光探针吡咯红与人血清白蛋白的结合常数（Kb = 4.7×10⁵ M⁻¹）和结合位点数（N = 2）。吡咯红对白蛋白的亲和力比8-苯胺基萘磺酸盐低20%，比另一种探针K35高2.4倍。因此，吡咯红对白蛋白的亲和力与苯胺基萘磺酸盐几乎相同，可被视为苯胺基萘磺酸盐的长波长“红色”类似物。
