Hou Xiaoli, Tong Xiaofei, Dong Wenjuan, Dong Chuan, Shuang Shaomin
Institute of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Mar;66(3):552-6. doi: 10.1016/j.saa.2006.03.031. Epub 2006 Apr 6.
A new synchronous fluorescence scan analysis was developed for the determination of HSA with high sensitivity with a triphenylmethane acid dye methyl blue as a fluorescence probe. When Deltalambda=140 nm, the synchronous fluorescence peak of methyl blue is located at 323 nm and the synchronous fluorescence intensity of the methyl blue is significantly increased in the presence of trace HSA due to the complex formed between methyl blue and HSA at pH 4.1. Under optimal conditions, the calibration graphs are linear over the range 0.03-266.0 and 266.0-665.0 microg mL(-1) for human serum albumin (HSA). Limit of determination were 0.03 microg mL(-1) for HSA. In the detection of HSA in human serum samples, this method gave values close the clinical data got from hospital.
开发了一种新的同步荧光扫描分析法，以三苯甲烷酸性染料甲基蓝作为荧光探针，用于高灵敏度地测定人血清白蛋白（HSA）。当Δλ=140nm时，甲基蓝的同步荧光峰位于323nm处，并且由于在pH 4.1条件下甲基蓝与HSA形成复合物，在痕量HSA存在下甲基蓝的同步荧光强度显著增加。在最佳条件下，人血清白蛋白（HSA）的校准曲线在0.03 - 266.0和266.0 - 665.0μg mL(-1)范围内呈线性。HSA的测定限为0.03μg mL(-1)。在检测人血清样品中的HSA时，该方法得到的值与从医院获得的临床数据接近。
