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[农杆菌的T-DNA通过细胞壁和细胞膜转移至植物细胞]

[Transfer of T-DNA from agrobacteria into plant cells through cell walls and membranes].

作者信息

Chumakov M I

机构信息

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov.

出版信息

Mol Gen Mikrobiol Virusol. 2001(1):13-29.

Abstract

Discusses probable routes of agrobacterial penetration through the plant integumental tissues, cell wall, and plant cell plasmodesma. Analyzes the contribution of extracellular structures of agrobacteria in penetration through barriers of a plant cell, primary contact (adhesion), and during DNA transfer from bacterial (E. coli, A. tumefaciens) to recipient (bacterial or plant) cells. Discusses the relationship between donor cell adhesion to recipient cell surface and the infectious and conjugation processes. Considers the probable role of piles in conjugative transfer of agrobacterial DNA through membranes of donor and recipient (bacterial and plant) cells. Analyzes the contribution of the plant cell cytoskeleton to T-DNA transfer. Suggests a model of transport of T-DNA-VirD2 complex and VirE2 proteins through independent channels consisting of vir-coded proteins.

摘要

讨论了农杆菌穿透植物体表组织、细胞壁和植物细胞胞间连丝的可能途径。分析了农杆菌细胞外结构在穿透植物细胞屏障、初次接触(黏附)以及从细菌(大肠杆菌、根癌农杆菌)向受体(细菌或植物)细胞进行DNA转移过程中的作用。讨论了供体细胞与受体细胞表面黏附与感染和接合过程之间的关系。考虑了菌毛在农杆菌DNA通过供体和受体(细菌和植物)细胞膜进行接合转移中的可能作用。分析了植物细胞细胞骨架对T-DNA转移的作用。提出了T-DNA-VirD2复合物和VirE2蛋白通过由vir编码蛋白组成的独立通道进行运输的模型。

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