Egwu I N, Eveland W C
Med Microbiol Immunol. 1979 May 15;167(2):107-15. doi: 10.1007/BF02123560.
In this study, evidence for in vitro uptake, invasion, and cytopathogonomic effects of normal and variant strains of B. canis on tissue culture, is presented. B. canis L-phase were penicillin-induced and these microorganisms produced revertants on penicillin-free media. Tissue culture (LLC-MK2) cells were divided into different normal and variant-infected groups (I-IV), including controls. Bright-field and electron microscopic observations indicated uptake of all the strains and recognizable host cell damage (CPE) to varying degrees. At 72 h after infection, the extent of damage by L-phase was the least (55.5% CPE). The L-phase-derived revertants resulted in 80% damage; this approximates the adverse effect of normal B. canis (85%). In addition to these gross changes, various structural abnormalities, including pyknosis, nuclear disorganization, vacuolation, and karyorrhexis, were apparent. The implications of these findings and the indirect role of the L-phase in brucellosis due to B. canis are discussed.
在本研究中，展示了犬布鲁氏菌正常菌株和变异菌株在组织培养中的体外摄取、侵袭及细胞病理效应的证据。犬布鲁氏菌L型是由青霉素诱导产生的，这些微生物在无青霉素培养基上产生回复体。将组织培养（LLC-MK2）细胞分为不同的正常感染组和变异感染组（I-IV组），包括对照组。明场和电子显微镜观察表明，所有菌株均可被摄取，并对宿主细胞造成不同程度的可识别损伤（细胞病变效应）。感染后72小时，L型造成的损伤程度最小（细胞病变效应为55.5%）。L型衍生的回复体导致80%的损伤；这接近正常犬布鲁氏菌的不良效应（85%）。除了这些大体变化外，还出现了各种结构异常，包括核固缩、核解体、空泡化和核碎裂。讨论了这些发现的意义以及L型在犬布鲁氏菌病中的间接作用。
