Bowser D V, Foster J W, Cooper M D
Am J Vet Res. 1975 Mar;36(3):303-8.
Brucella suis strain 3b and Brucella canis strain RM-6-66 were grown on tryptose agar, and aqueous ether extracts were prepared from the cells. The aqueous phase was clarified by centrifugation for a total of 10 hours at 144,700 times g and fractionated with gels and with diethylaminoethyl (DEAE) cellulose. Two fractions from B suis and 1 fraction from B canis were reactive in the indirect hemagglutination test. The sensitizing ability was destroyed by pronase, but was unaffected by ribonuclease or deoxyribonuclease. Apparently, protein or one of its subunits has an important role in antigenic specificity relating to indirect hemagglutination activity. Results in immunoelectrophoretic analysis indicated at least 22 antigens were in the ultracentrifugal supernate (144,700 times g) of B suis and 31 antigens were in a similar supernate of B canis. Results in immunodiffusion and agglutination absorption studies indicated a close relationship existed between B suis and B canis.
猪布鲁氏菌3b菌株和犬布鲁氏菌RM-6-66菌株在胰蛋白胨琼脂上培养，然后从细胞中制备水相乙醚提取物。水相通过在144,700倍重力下总共离心10小时进行澄清，并用凝胶和二乙氨基乙基（DEAE）纤维素进行分级分离。来自猪布鲁氏菌的两个级分和来自犬布鲁氏菌的一个级分在间接血凝试验中呈阳性反应。致敏能力被链霉蛋白酶破坏，但不受核糖核酸酶或脱氧核糖核酸酶的影响。显然，蛋白质或其亚基之一在与间接血凝活性相关的抗原特异性中起重要作用。免疫电泳分析结果表明，猪布鲁氏菌的超速离心上清液（144,700倍重力）中至少有22种抗原，犬布鲁氏菌的类似上清液中有31种抗原。免疫扩散和凝集吸收研究结果表明，猪布鲁氏菌和犬布鲁氏菌之间存在密切关系。
