Zwijnenburg P J, van der Poll T, Florquin S, van Deventer S J, Roord J J, van Furth A M
Department of Pediatrics, University Hospital Vrije Universiteit, and Department of Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ, Amsterdam, The Netherlands.
J Infect Dis. 2001 Apr 1;183(7):1143-6. doi: 10.1086/319271. Epub 2001 Mar 1.
Effective laboratory animal models of bacterial meningitis are needed to unravel the pathophysiology of this disease. Previous models have failed to simulate human meningitis by using a directly intracerebral route of infection. Hyaluronidase is a virulence factor of Streptococcus pneumoniae. In this study, a novel model of murine meningitis is described. Intranasal administration of S. pneumoniae with hyaluronidase induced meningitis in 50% of inoculated mice, as defined by a positive cerebrospinal fluid (CSF) culture and an inflammatory infiltrate in the meninges. None of the mice inoculated without hyaluronidase developed meningitis. Hyaluronidase was found to facilitate pneumococcal invasion of the bloodstream after colonization of the upper respiratory tract. Meningitis was characterized by pleocytosis of CSF and the induction of proinflammatory cytokines and CXC chemokines in brain tissue. These results indicate that this murine model mimics important features of human disease and allow for the use of this model for studying issues related to the pathophysiology and the treatment of pneumococcal meningitis.
需要有效的细菌性脑膜炎实验动物模型来阐明这种疾病的病理生理学。以前的模型未能通过直接脑内感染途径模拟人类脑膜炎。透明质酸酶是肺炎链球菌的一种毒力因子。在本研究中，描述了一种新型的小鼠脑膜炎模型。经鼻给予肺炎链球菌和透明质酸酶，50% 的接种小鼠发生脑膜炎，定义为脑脊液（CSF）培养阳性且脑膜有炎性浸润。未接种透明质酸酶的小鼠均未发生脑膜炎。发现透明质酸酶在上呼吸道定植后促进肺炎球菌侵入血流。脑膜炎的特征是脑脊液细胞增多以及脑组织中促炎细胞因子和 CXC 趋化因子的诱导。这些结果表明，该小鼠模型模拟了人类疾病的重要特征，并可用于研究与肺炎球菌性脑膜炎的病理生理学和治疗相关的问题。
