Kent N A, Karabetsou N, Politis P K, Mellor J
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.
Genes Dev. 2001 Mar 1;15(5):619-26. doi: 10.1101/gad.190301.
Isw1p and Isw2p are budding yeast homologs of the Drosophila ISWI chromatin-remodeling ATPase. Using indirect-end-label and chromatin immunoprecipitation analysis, we show both independent and cooperative Isw1p- and Isw2p-mediated positioning of short nucleosome arrays in gene-regulatory elements at a variety of transcription units in vivo. We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth.
Isw1p和Isw2p是果蝇ISWI染色质重塑ATP酶在芽殖酵母中的同源物。通过间接末端标记和染色质免疫沉淀分析，我们发现Isw1p和Isw2p在体内多种转录单位的基因调控元件中，对短核小体阵列的定位既具有独立性又存在协同作用。我们提供的证据表明，酵母的两种ISWI复合物都调节对饥饿的发育反应，对于Isw2p而言，不同DNA结合蛋白的招募控制减数分裂和单倍体侵袭性生长。
