Watakabe A, Fujita H, Hayashi M, Yamamori T
National Institute for Basic Biology, Okazaki, Japan.
J Neurochem. 2001 Mar;76(5):1455-64. doi: 10.1046/j.1471-4159.2001.00177.x.
We applied a differential display PCR technique to isolate molecules that are area-specific in expression in the primate neocortex, and found that growth/differentiation factor 7 (GDF7), a member of the bone morphogenetic protein (BMP)/transforming growth factor (TGF) beta super-family, is preferentially expressed in the primary motor area of African green monkeys (Cercopithecus aethiops). We proved that GDF7 is 10 times more abundant in the motor cortex than in the visual cortex by northern blotting and quantitative RT-PCR. When we examined the neocortex of closely related rhesus monkeys (Macaca mulatta), GDF7 was also most abundant in the motor cortex, although the regional difference was reduced to 3-fold. This differential expression pattern was observed in both newborn and infant rhesus monkeys. We found that several type I/II receptors of BMP, candidates of the receptors for GDF7, are uniformly expressed in the mature neocortex. The unique expression pattern of GDF7 suggests that it may play an active role in the motor area of the primate neocortex.
我们应用差异显示PCR技术分离在灵长类动物新皮层中表达具有区域特异性的分子，发现骨形态发生蛋白（BMP）/转化生长因子（TGF）β超家族成员生长/分化因子7（GDF7）在非洲绿猴（Cercopithecus aethiops）的初级运动区优先表达。通过Northern印迹和定量RT-PCR，我们证明GDF7在运动皮层中的丰度比视觉皮层高10倍。当我们检查亲缘关系密切的恒河猴（Macaca mulatta）的新皮层时，GDF7在运动皮层中也最为丰富，尽管区域差异降至3倍。在新生和幼年恒河猴中均观察到这种差异表达模式。我们发现BMP的几种I/II型受体，即GDF7受体的候选者，在成熟新皮层中均一表达。GDF7独特的表达模式表明它可能在灵长类动物新皮层的运动区发挥积极作用。
