Davidson A J, Postlethwait J H, Yan Y L, Beier D R, van Doren C, Foernzler D, Celeste A J, Crosier K E, Crosier P S
Department of Molecular Medicine, School of Medicine, University of Auckland, Auckland, New Zealand.
Genome Res. 1999 Feb;9(2):121-9.
The Growth/differentiation factor (Gdf) 5, 6, 7 genes form a closely related subgroup belonging to the TGF-beta superfamily. In zebrafish, there are three genes that belong to the Gdf5, 6, 7 subgroup that have been named radar, dynamo, and contact. The genes radar and dynamo both encode proteins most similar to mouse GDF6. The orthologous identity of these genes on the basis of amino acid similarities has not been clear. We have identified gdf7, a fourth zebrafish gene belonging to the Gdf5, 6, 7 subgroup. To assign correct orthologies and to investigate the evolutionary relationships of the human, mouse, and zebrafish Gdf5, 6, 7 subgroup, we have compared genetic map positions of the zebrafish and mammalian genes. We have mapped zebrafish gdf7 to linkage group (LG) 17, contact to LG9, GDF6 to human chromosome (Hsa) 8 and GDF7 to Hsa2p. The radar and dynamo genes have been localized previously to LG16 and LG19, respectively. A comparison of syntenies shared among human, mouse, and zebrafish genomes indicates that gdf7 is the ortholog of mammalian GDF7/Gdf7. LG16 shares syntenic relationships with mouse chromosome (Mmu) 4, including Gdf6. Portions of LG16 and LG19 appear to be duplicate chromosomes, thus suggesting that radar and dynamo are both orthologs of Gdf6. Finally, the mapping data is consistent with contact being the zebrafish ortholog of mammalian GDF5/Gdf5.
生长/分化因子（Gdf）5、6、7基因形成了一个属于转化生长因子-β超家族的密切相关亚组。在斑马鱼中，有三个属于Gdf5、6、7亚组的基因，分别命名为radar、dynamo和contact。radar和dynamo基因编码的蛋白质与小鼠GDF6最为相似。基于氨基酸相似性，这些基因的直系同源关系尚不清楚。我们鉴定出了gdf7，这是斑马鱼中属于Gdf5、6、7亚组的第四个基因。为了确定正确的直系同源关系，并研究人类、小鼠和斑马鱼Gdf5、6、7亚组的进化关系，我们比较了斑马鱼和哺乳动物基因的遗传图谱位置。我们已将斑马鱼gdf7定位到连锁群（LG）17，contact定位到LG9，GDF6定位到人类染色体（Hsa）8，GDF7定位到Hsa2p。radar和dynamo基因先前已分别定位到LG16和LG19。对人类、小鼠和斑马鱼基因组之间共享的同线性进行比较表明，gdf7是哺乳动物GDF7/Gdf7的直系同源基因。LG16与小鼠染色体（Mmu）4具有同线性关系，包括Gdf6。LG16和LG19的部分区域似乎是重复染色体，因此表明radar和dynamo都是Gdf6的直系同源基因。最后，图谱数据与contact是哺乳动物GDF5/Gdf5的斑马鱼直系同源基因这一结论一致。
