Upf1p、Nmd2p和Upf3p调节含无义密码子的mRNA以及野生型mRNA的脱帽和核酸外切酶降解。
Upf1p, Nmd2p, and Upf3p regulate the decapping and exonucleolytic degradation of both nonsense-containing mRNAs and wild-type mRNAs.
作者信息
He F, Jacobson A
机构信息
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0122, USA.
出版信息
Mol Cell Biol. 2001 Mar;21(5):1515-30. doi: 10.1128/MCB.21.5.1515-1530.2001.
Abstract
In Saccharomyces cerevisiae, rapid degradation of nonsense-containing mRNAs requires the decapping enzyme Dcp1p, the 5'-to-3' exoribonuclease Xrn1p, and the three nonsense-mediated mRNA decay (NMD) factors, Upf1p, Nmd2p, and Upf3p. To identify specific functions for the NMD factors, we analyzed the mRNA decay phenotypes of yeast strains containing deletions of DCP1 or XRN1 and UPF1, NMD2, or UPF3. Our results indicate that Upf1p, Nmd2p, and Upf3p regulate decapping and exonucleolytic degradation of nonsense-containing mRNAs. In addition, we show that these factors also regulate the same processes in the degradation of wild-type mRNAs. The participation of the NMD factors in general mRNA degradation suggests that they may regulate an aspect of translation termination common to all transcripts.
摘要
在酿酒酵母中，含无义密码子的mRNA的快速降解需要去帽酶Dcp1p、5'至3'外切核糖核酸酶Xrn1p以及三个无义介导的mRNA降解（NMD）因子Upf1p、Nmd2p和Upf3p。为了确定NMD因子的具体功能，我们分析了缺失DCP1或XRN1以及UPF1、NMD2或UPF3的酵母菌株的mRNA降解表型。我们的结果表明，Upf1p、Nmd2p和Upf3p调节含无义密码子的mRNA的去帽和核酸外切酶降解。此外，我们表明这些因子在野生型mRNA的降解中也调节相同的过程。NMD因子参与一般mRNA降解表明它们可能调节所有转录本共有的翻译终止的一个方面。
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