Yang C, Mora S, Ryder J W, Coker K J, Hansen P, Allen L A, Pessin J E
Department of Physiology and Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA.
Mol Cell Biol. 2001 Mar;21(5):1573-80. doi: 10.1128/MCB.21.5.1573-1580.2001.
To investigate the physiological function of the VAMP3 vesicle SNARE (v-SNARE) isoform in the regulation of GLUT4 vesicle trafficking, we generated homozygotic VAMP3 null mice by targeted gene disruption. The VAMP3 null mice had typical growth rate and weight gain, with normal maintenance of fasting serum glucose and insulin levels. Analysis of glucose disposal and insulin sensitivity demonstrated normal insulin and glucose tolerance, with no evidence for insulin resistance. Insulin stimulation of glucose uptake in isolated primary adipocytes was essentially the same for the wild-type and VAMP3 null mice. Similarly, insulin-, hypoxia-, and exercise-stimulated glucose uptake in isolated skeletal muscle did not differ significantly. In addition, other general membrane trafficking events including phagocytosis, pinocytosis, and transferrin receptor recycling were also found to be unaffected in the VAMP3 null mice. Taken together, these data demonstrate that VAMP3 function is not necessary for either regulated GLUT4 translocation or general constitutive membrane recycling.
为了研究VAMP3囊泡可溶性N - 乙基马来酰亚胺敏感因子附着蛋白受体（v - SNARE）亚型在调节葡萄糖转运蛋白4（GLUT4）囊泡运输中的生理功能，我们通过靶向基因敲除生成了纯合VAMP3基因缺失小鼠。VAMP3基因缺失小鼠具有典型的生长速率和体重增加，空腹血清葡萄糖和胰岛素水平维持正常。对葡萄糖处置和胰岛素敏感性的分析表明胰岛素和葡萄糖耐受性正常，没有胰岛素抵抗的证据。野生型和VAMP3基因缺失小鼠的分离原代脂肪细胞中胰岛素刺激的葡萄糖摄取基本相同。同样，分离的骨骼肌中胰岛素、缺氧和运动刺激的葡萄糖摄取也没有显著差异。此外，还发现VAMP3基因缺失小鼠的其他一般膜运输事件，包括吞噬作用、胞饮作用和转铁蛋白受体循环均未受影响。综上所述，这些数据表明VAMP3功能对于调节GLUT4易位或一般组成性膜循环均非必需。
