Struijk D G, Douma C E, Krediet R T, Zweers M M
Department of Nephrology, Academic Medical Center, University of Amsterdam. Dianet Foundation, Utrecht, The Netherlands.
Nephrol Dial Transplant. 2001 Mar;16(3):661-3. doi: 10.1093/ndt/16.3.661.
It is unclear whether nitric oxide (NO) is important in regulating peritoneal transport during non-infected peritoneal dialysis.
In 13 rabbits, 250 mg/l L-arginine, a substrate for NO synthesis, was added to a 3.86% glucose dialysis solution. N:(G)-monomethyl-L-arginine (L-NMMA) 25 mg/1, an inhibitor of NO synthase, was added to the dialysate in 10 rabbits. Standard peritoneal permeability analyses in rabbits were used to analyse the effects of these interventions on solute transport during 1-h dwells. The addition of 4.5 mg/l nitroprusside to the dialysate in five rabbits was used for validation of this model.
Nitroprusside caused an 86% (48-233%) increase in albumin clearance, which is similar to the nitroprusside-induced increase found in humans (70%). Contrary to human studies, no effect was found on the mass transfer area coefficient (MTAC) of urea and creatinine, or on glucose absorption. L-Arginine did not affect either the MTAC of urea and creatinine, or the absorption of glucose. Peritoneal albumin clearance increased 18% (-24 to 609%). This resembles the NO-mediated effects of nitroprusside. Addition of L-NMMA caused no change in the solute transport rate.
The rabbit dialysis model can be used for analysing the effects of interventions on peritoneal permeability characteristics, although the rabbit peritoneal membrane is probably less sensitive to NO compared with that of humans. L-Arginine-induced effects are similar to those of nitroprusside, which suggests that these effects possibly are mediated by NO. As L-NMMA did not affect peritoneal transport, it is unlikely that NO is involved in the regulation of peritoneal permeability in rabbits.
尚不清楚一氧化氮（NO）在非感染性腹膜透析过程中调节腹膜转运是否重要。
在13只兔子中，将250mg/L的L-精氨酸（一种NO合成的底物）添加到3.86%的葡萄糖透析液中。在10只兔子的透析液中添加25mg/1的N:（G）-单甲基-L-精氨酸（L-NMMA），一种NO合酶抑制剂。采用兔标准腹膜通透性分析来分析这些干预措施对1小时驻留期间溶质转运的影响。在5只兔子的透析液中添加4.5mg/L的硝普钠用于验证该模型。
硝普钠使白蛋白清除率增加了86%（48 - 233%），这与在人类中发现的硝普钠诱导的增加（70%）相似。与人体研究相反，未发现对尿素和肌酐的传质面积系数（MTAC）或葡萄糖吸收有影响。L-精氨酸对尿素和肌酐的MTAC或葡萄糖吸收均无影响。腹膜白蛋白清除率增加了18%（-24至609%）。这类似于硝普钠的NO介导效应。添加L-NMMA后溶质转运速率没有变化。
兔透析模型可用于分析干预措施对腹膜通透性特征的影响，尽管兔腹膜对NO的敏感性可能低于人类。L-精氨酸诱导的效应与硝普钠相似，这表明这些效应可能由NO介导。由于L-NMMA不影响腹膜转运，NO不太可能参与兔腹膜通透性的调节。
