Preuss H G, Tourkantonis A, Hsu C H, Shim P S, Barzyk P, Tio F, Schreiner G E
Lab Invest. 1975 Mar;32(3):286-94.
Metabolic and morphologic changes occurred in the kidneys of rats within 3 hours after inciting acute tubular necrosis by completely clamping the renal blood supply, by intramuscular injections of glycerol, and by subcutaneous injections of HgC12. Although the initial trend was for p-aminohippurate and tetraethylammonium transport to decrease and for oxygen consumption, ammonia production, and gluconeogenesis to increase after glycerol, all of these parameters changed in opposite directions after renal pedicle clamping and after subcutaneous HgC12 (4.7 mg. per kg;). In addition, early morphologic changes in glycerol-injected rats differed from those seen with pedicle clamping and low dose HgC12. With high dose HgC12 (25 mg. per kg.), the metabolic and morphologic changes were somewhere in between those seen with the other insults. Coinciding with early metabolic and morphologic changes, cardiac output and renal blood flow decreased soon after the glycerol was given. On the basis of our findings, we cannot ascribe all of the early metabolic and morphologic changes in the glycerol model to ischemia, and we postulate that the circulating heme proteins may be nephrotoxic to ischemic renal tissue.
通过完全夹闭肾血流、肌肉注射甘油以及皮下注射HgC12诱导大鼠急性肾小管坏死，3小时内大鼠肾脏发生了代谢和形态学变化。尽管在注射甘油后，对氨基马尿酸和四乙铵转运最初呈下降趋势，而氧消耗、氨生成和糖异生呈增加趋势，但在夹闭肾蒂和皮下注射HgC12（4.7毫克/千克）后，所有这些参数均呈相反方向变化。此外，注射甘油的大鼠早期形态学变化与夹闭肾蒂和低剂量HgC12所致变化不同。高剂量HgC12（25毫克/千克）时，代谢和形态学变化介于其他损伤所见变化之间。与早期代谢和形态学变化一致，注射甘油后不久心输出量和肾血流量下降。根据我们的研究结果，我们不能将甘油模型中的所有早期代谢和形态学变化都归因于缺血，我们推测循环中的血红素蛋白可能对缺血性肾组织具有肾毒性。
