Zimmermann R, Schmidt S, Zingsem J, Glaser A, Weisbach V, Ruf A, Eckstein R
Department of Transfusion Medicine and Hemostaseology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany.
Transfusion. 2001 Feb;41(2):236-42. doi: 10.1046/j.1537-2995.2001.41020236.x.
The effect of gamma radiation on single-donor apheresis platelet concentrates (SDPs) has been elucidated only incompletely. The only existing report on the function of SDPs stored in the irradiated state found a deterioration in the in vitro aggregability at the end of shelf life in SDPs divided before irradiation with 1500 cGy.
The in vitro properties of platelets were examined in four series of irradiated and control platelets, each obtained from the same 15 donors. Irradiation with 3000 cGy was performed on Days 0, 3, and 5. Cellular content, aggregability by ADP alone or ADP and epinephrine, spontaneous and induced CD62 expression, beta-thromboglobulin release, glucose consumption, lactate production, and pH were measured immediately after preparation and on Days 3 and 5 after donation.
Comparable in vitro properties were measured in irradiated and control platelets, whether irradiation was performed on Day 3 or Day 5. However, in platelets irradiated on Day 0, we found a significantly better in vitro aggregability by 20 microM: ADP immediately after irradiation and by 10 microM: ADP and 2 microM: epinephrine at the end of shelf life than was found in the other groups (Day 5 results: Day 0 irradiation: 75 +/- 32%; Day 3 irradiation: 45 +/- 45%; Day 5 irradiation: 47 +/- 41%; control: 40 +/- 24%; p<0.05).
Gamma radiation had no adverse effect on platelet quality in extremely WBC-reduced SDPs. On the contrary, a slight, but significantly better in vitro aggregability was found in SDPs irradiated before storage than in platelets irradiated later during storage and in unirradiated platelets. This increased in vitro aggregability persisted until the end of shelf life.
γ射线对单供体单采血小板浓缩物（SDPs）的影响尚未完全阐明。关于辐照状态下储存的SDPs功能的唯一现有报告发现，在1500 cGy辐照前分割的SDPs在保质期结束时体外聚集性下降。
在四组辐照和对照血小板中检测血小板的体外特性，每组均取自相同的15名供体。在第0天、第3天和第5天进行3000 cGy的辐照。制备后立即以及献血后第3天和第5天测量细胞含量、单独ADP或ADP与肾上腺素的聚集性、自发和诱导的CD62表达、β-血小板球蛋白释放、葡萄糖消耗、乳酸生成和pH值。
无论辐照是在第3天还是第5天进行，辐照和对照血小板的体外特性相当。然而，在第0天辐照的血小板中，我们发现辐照后立即用20μM ADP以及保质期结束时用10μM ADP和2μM肾上腺素检测时，其体外聚集性明显优于其他组（第5天结果：第0天辐照：75±32%；第3天辐照：45±45%；第5天辐照：47±41%；对照：40±24%；p<0.05）。
γ射线对极低白细胞SDPs的血小板质量无不良影响。相反，储存前辐照的SDPs比储存后期辐照的血小板和未辐照的血小板具有轻微但明显更好的体外聚集性。这种体外聚集性的增加一直持续到保质期结束。
