Sweeney J D, Holme S, Moroff G
American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Transfusion. 1994 Sep;34(9):779-83. doi: 10.1046/j.1537-2995.1994.34994378279.x.
There are conflicting data on the effect of irradiation and subsequent storage on the quality of platelet components.
The retention of platelet properties during storage of gamma-irradiated apheresis suspensions was studied in 22 apheresis components obtained on a cell separator with a specialized centrifugation chamber. Immediately after collection, each suspension was divided equally into two 1-L polyolefin containers. On Day 1 (n = 12) and Day 3 (n = 10) one of each pair of suspension containers was gamma radiated with 2500 cGy. All platelet suspensions were stored for 5 days at 20 to 24 degrees C. Samples were drawn on Day 5 from each of the 22 pairs of containers for evaluation of an array of in vitro properties. Samples were taken from 10 pairs of containers for platelet labeling with either 51Cr or 111In for subsequent transfusion and concurrent in vivo measurement of recovery and survival. Posttransfusion samples were drawn after 24 hours for ex vivo whole blood aggregation.
Comparable in vitro and in vivo properties were measured in irradiated and control platelets, whether irradiation was performed on Day 1 or Day 3. The mean +/- 1 SD in vivo recovery and survival time for controls and platelets irradiated on Day 1 was 52 +/- 14 percent and 146 +/- 34 hours and 51 +/- 7 percent and 147 +/- 36 hours, respectively. For Day 3 irradiation, the values were 46 +/- 12 percent and 150 +/- 60 hours and 47 +/- 9 percent and 151 +/- 53 hours, respectively. A small, but measurable adverse effect of irradiation on ex vivo platelet aggregation was present.
These data indicate that storage of apheresis platelets after gamma radiation is without clinically significant, demonstrably adverse effects on platelet quality.
关于辐照及随后储存对血小板成分质量的影响，存在相互矛盾的数据。
在配备专门离心腔的血细胞分离机上采集了22份单采成分，研究了γ辐照单采悬浮液储存期间血小板特性的保留情况。采集后，每份悬浮液立即等分为两个1升聚烯烃容器。在第1天（n = 12）和第3天（n = 10），每对悬浮液容器中的一个接受2500 cGy的γ辐射。所有血小板悬浮液均在20至24摄氏度下储存5天。在第5天从22对容器中的每一对抽取样本，以评估一系列体外特性。从10对容器中取样，用51Cr或111In对血小板进行标记，以便随后输血并同时进行体内回收率和存活率测量。输血后24小时抽取样本进行体外全血凝集试验。
无论是在第1天还是第3天进行辐照，辐照血小板和对照血小板的体外和体内特性相当。对照血小板以及在第1天辐照的血小板的平均±1标准差体内回收率和存活时间分别为52±14%和146±34小时以及51±7%和147±36小时。对于第3天辐照，相应数值分别为46±12%和150±60小时以及47±9%和151±53小时。辐照对体外血小板凝集有轻微但可测量的不利影响。
这些数据表明，γ辐射后单采血小板的储存对血小板质量无临床显著的明显不利影响。
