Liljedahl M, Maeda Y, Colanzi A, Ayala I, Van Lint J, Malhotra V
Biology Department, University of California, San Diego, La Jolla, CA 92093, USA.
Cell. 2001 Feb 9;104(3):409-20. doi: 10.1016/s0092-8674(01)00228-8.
When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface.
当蛋白激酶D的激酶失活形式（PKD-K618N）在HeLa细胞中表达时，它定位于反式高尔基体网络（TGN）并导致广泛的管状化。发现在含有PKD-K618N的管中有运往质膜的货物，但这些管并未从TGN脱离。结果，货物从TGN向质膜的转运受到抑制。我们还证明了在稳定表达低水平PKD-K618N的细胞中，含货物的管从TGN形成并随后脱离。我们的结果表明，PKD调节了正在运往细胞表面的运输载体从TGN的裂变。
