Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG) and UPF, Barcelona, Spain.
EMBO J. 2012 Oct 17;31(20):3976-90. doi: 10.1038/emboj.2012.235. Epub 2012 Aug 21.
We have isolated a membrane fraction enriched in a class of transport carriers that form at the trans Golgi network (TGN) and are destined for the cell surface in HeLa cells. Protein kinase D (PKD) is required for the biogenesis of these carriers that contain myosin II, Rab6a, Rab8a, and synaptotagmin II, as well as a number of secretory and plasma membrane-specific cargoes. Our findings reveal a requirement for myosin II in the migration of these transport carriers but not in their biogenesis per se. Based on the cargo secreted by these carriers we have named them CARTS for CARriers of the TGN to the cell Surface. Surprisingly, CARTS are distinct from the carriers that transport vesicular stomatitis virus (VSV)-G protein and collagen I from the TGN to the cell surface. Altogether, the identification of CARTS provides a valuable means to understand TGN to cell surface traffic.
我们已经分离出富含一类运输载体的膜部分，这些载体在 HeLa 细胞中形成于反高尔基网络 (TGN)，并注定要到达细胞表面。蛋白激酶 D (PKD) 是这些载体生物发生所必需的，这些载体包含肌球蛋白 II、Rab6a、Rab8a 和突触结合蛋白 II ，以及许多分泌和质膜特异性货物。我们的发现揭示了肌球蛋白 II 在这些运输载体迁移中的必要性，但本身并不是其生物发生所必需的。基于这些载体分泌的货物，我们将它们命名为 CARTS，即从 TGN 到细胞表面的运输载体。令人惊讶的是，CARTS 与从 TGN 运输囊泡性口炎病毒 (VSV)-G 蛋白和胶原蛋白 I 的载体不同。总的来说，CARTS 的鉴定为理解 TGN 到细胞表面的运输提供了一种有价值的手段。
