Ming G, Henley J, Tessier-Lavigne M, Song H, Poo M
Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA.
Neuron. 2001 Feb;29(2):441-52. doi: 10.1016/s0896-6273(01)00217-3.
Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca(2+) during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca(2+) and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.
对培养的非洲爪蟾脊髓神经元进行短暂的电刺激，会导致生长锥在吸引或排斥导向线索梯度诱导下的转向反应发生显著改变。Netrin-1诱导的吸引力增强，髓磷脂相关糖蛋白（MAG）或髓磷脂膜碎片诱导的排斥反应则转变为吸引。该效应在电刺激期间需要细胞外Ca(2+)的存在，并且似乎是由细胞质Ca(2+)和cAMP的升高介导的。因此，电活动可能会影响发育中神经元的轴突寻路，间歇性电刺激可能对促进损伤后的神经再生有效。
