在体外流动条件下，二磷酸腺苷激活的血小板与人脑微血管内皮细胞的黏附是通过糖蛋白IIb/IIIa介导的。
Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa.
作者信息
Tanahashi N, Fukuuchi Y, Tomita M, Tomita Y, Inoue K, Satoh H, Abe T
机构信息
Department of Neurology, School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160-8582, Tokyo, Japan.
出版信息
Neurosci Lett. 2001 Mar 23;301(1):33-6. doi: 10.1016/s0304-3940(01)01608-1.
Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK-029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets to human brain microvascular endothelial cells (HBEC) in vitro. HBECs were cultured on a coverglass and put in the observation chamber of VEC microscopy. Then, activated platelets by adenosine diphosphate (ADP) (2 microM) were perfused over HBEC at a low shear rate of 10 s(-1) for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 microM) plus TAK-029 (GPIIb/IIIa antagonist; 1 microM) was perfused over HBEC for 30 min and washed out. Anti-GPIbalpha antibody (GUR20-5) did not inhibit adhesion of ADP-activated platelets to HBEC. The above results showed adhesion of ADP-activated platelets to HBEC under flow in vitro is mediated via GPIIb/IIIa
采用视频增强对比度（VEC）显微镜，我们研究了TAK - 029（糖蛋白IIb/IIIa拮抗剂）在体外是否抑制活化血小板与人脑微血管内皮细胞（HBEC）的黏附。将HBEC培养在盖玻片上，并置于VEC显微镜的观察室中。然后，以10 s(-1)的低剪切速率将由二磷酸腺苷（ADP）（2微摩尔）活化的血小板灌注到HBEC上30分钟，然后冲洗掉。血小板直接黏附于HBEC。然而，当含有ADP（2微摩尔）加TAK - 029（糖蛋白IIb/IIIa拮抗剂；1微摩尔）的富血小板血浆灌注到HBEC上30分钟然后冲洗掉时，血小板与HBEC的黏附受到抑制。抗糖蛋白Ibalpha抗体（GUR20 - 5）不抑制ADP活化血小板与HBEC的黏附。上述结果表明，体外流动状态下ADP活化血小板与HBEC的黏附是通过糖蛋白IIb/IIIa介导的
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