Võikar V, Kõks S, Vasar E, Rauvala H
Laboratory of Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, PO Box 56, Viikinkaari 5, FIN-00014 Helsinki, Finland.
Physiol Behav. 2001 Jan;72(1-2):271-81. doi: 10.1016/s0031-9384(00)00405-4.
The present study was aimed at establishing behavioral differences between three inbred mouse strains (129S2/SvHsd, C57BL/6JOlaHsd, FVB/NHsd) and two F1 hybrid lines derived from them (129 x C57BL/6 and 129 x FVB). The choice of the given strains was based on the frequent use of these mice in transgenic research. For the behavioral phenotyping, we employed a test battery consisting of the following models: elevated plus-maze (EPM), open field (OF), light-dark exploration, spontaneous locomotor activity, rota-rod (RR), Porsolt's forced-swimming test (FST), and Morris water task. Significant variations between the strains were established in all tests. Anxiety-like behavior was more pronounced in the 129S2/Sv and 129 x C57BL/6 mice, the FVB/N mice were spontaneously hyperactive, the best coordination ability was demonstrated by the C57BL/6 and 129 x C57BL/6 groups. A good performance in the learning test was established in both hybrid lines and the 129S2/Sv mice, whereas the well-known visual impairment of the FVB strain was confirmed by low performance in spatial and non-spatial tasks. Differences related to the gender were revealed occasionally; most importantly, 129 x C57BL/6 males had a higher anxiety level than their female counterparts in the EPM. Several other gender dissociations suggest the strain and task specificity. In conclusion, we would like to highlight the importance of the genetic background and gender of mice for the molecular biological and pharmacological studies and also the need for well-established testing protocols to obtain wide information at the first stage of behavioral screening of genetically modified mice.
本研究旨在确定三种近交系小鼠品系（129S2/SvHsd、C57BL/6JOlaHsd、FVB/NHsd）以及由它们衍生的两个F1杂交系（129×C57BL/6和129×FVB）之间的行为差异。选择这些特定品系是基于它们在转基因研究中的频繁使用。对于行为表型分析，我们采用了一组测试，包括以下模型：高架十字迷宫（EPM）、旷场试验（OF）、明暗箱探索试验、自发运动活动、转棒试验（RR）、波索尔特强迫游泳试验（FST）和莫里斯水迷宫任务。在所有测试中均确定了品系之间的显著差异。129S2/Sv和129×C57BL/6小鼠的焦虑样行为更为明显，FVB/N小鼠自发活动亢进，C57BL/6和129×C57BL/6组表现出最佳的协调能力。两个杂交系和129S2/Sv小鼠在学习测试中表现良好，而FVB品系众所周知的视觉障碍通过其在空间和非空间任务中的低表现得到证实。偶尔会发现与性别相关的差异；最重要的是，在EPM中，129×C57BL/6雄性小鼠的焦虑水平高于雌性。其他一些性别差异表明了品系和任务的特异性。总之，我们想强调小鼠的遗传背景和性别对于分子生物学和药理学研究的重要性，以及在转基因小鼠行为筛选的第一阶段需要完善的测试方案以获取广泛信息。
