Wakugawa M, Nakamura K, Akatsuka M, Kim S S, Yamada Y, Kawasaki H, Tamaki K, Furue M
Department of Dermatology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan.
J Dermatol Sci. 2001 Apr;25(3):229-35. doi: 10.1016/s0923-1811(00)00148-1.
CC chemokines and their ligands, CC chemokine receptors (CCRs), play an important role in the process of inflammation such as trafficking and activating inflammatory cells. CCR3 is known to be a ligand for CC chemokines such as RANTES, eotaxin and monocyte-chemotactic protein-3 (MCP-3). In this study we examined the expression of CCR3 in cultured normal human keratinocytes (KCs). CCR3 protein and mRNA expressions were detected in cultured normal KCs by flow cytometric (FACS) analysis and reverse-transcription-polymerase chain reaction (RT-PCR) analysis. FACS analysis demonstrated that CCR3 expression on KCs was significantly upregulated when the cells were cultured with RANTES, but not with eotaxin, IL-4 or interferon-gamma. RT-PCR analysis revealed that CCR3 mRNA was detectable in normal KCs. We also examined the immunoreactivity of CCR3 in normal skin and inflammatory skin lesions. CCR3 was detected weakly in epidermis of normal skin, while strong immunoreactivity for CCR3 was seen in epidermis of inflammatory skin lesions such as atopic dermatitis. These results suggest that CCR3 is constitutively expressed on KCs and is involved in inflammatory modulation. RANTES may regulate the function of KCs through CCR3.
CC趋化因子及其配体，即CC趋化因子受体（CCR），在炎症过程中发挥重要作用，如运输和激活炎症细胞。已知CCR3是RANTES、嗜酸性粒细胞趋化因子和单核细胞趋化蛋白-3（MCP-3）等CC趋化因子的配体。在本研究中，我们检测了培养的正常人角质形成细胞（KC）中CCR3的表达。通过流式细胞术（FACS）分析和逆转录聚合酶链反应（RT-PCR）分析，在培养的正常KC中检测到CCR3蛋白和mRNA表达。FACS分析表明，当细胞与RANTES一起培养时，KC上的CCR3表达显著上调，但与嗜酸性粒细胞趋化因子、IL-4或干扰素-γ一起培养时则不然。RT-PCR分析显示，在正常KC中可检测到CCR3 mRNA。我们还检测了正常皮肤和炎症性皮肤病变中CCR3的免疫反应性。在正常皮肤的表皮中CCR3检测较弱，而在特应性皮炎等炎症性皮肤病变的表皮中可见CCR3的强免疫反应性。这些结果表明，CCR3在KC上组成性表达，并参与炎症调节。RANTES可能通过CCR3调节KC的功能。
