Isoardo G, Ferrero B, Barbero P, Cucci A, Oggero A, Pipieri A, Ricci A, Verdun E, Bergamasco B, Durelli L
Clinica Neurologica I, Dipartimento di Neuroscienze, Università di Torino, Italy.
Acta Neurol Scand. 2001 Mar;103(3):180-7. doi: 10.1034/j.1600-0404.2001.103003180.x.
To determine threshold titers and diagnostic accuracy of anti-GM1 and anti-sulfatide antibodies (Ab) for autoimmune polyneuropathies (PN) in overall and for particular subtypes of them.
In this study 84 PN patients, 120 epileptics and 93 healthy controls' sera were tested by enzyme-linked immunosorbent assay for autoAbs and results confirmed by thin-layer chromatography. Frequencies of positive patients at increasing cut-off were compared to determine threshold titers. Accuracy was determined by Receiver Operator Characteristic analysis.
A 1:2,000 titer for IgM anti-GM1 and a 1:4,000 titer for IgM anti-sulfatide Ab resulted to be threshold titers for autoimmune PN in overall. IgM anti-GM1 and anti-sulfatide Ab had low discriminating capacity between autoimmune PN and other PN, but good discriminating capacity between motor neuropathy (for anti-GM1 Ab) or PN in IgM-paraproteinemia or chronic painful sensory axonal PN (for anti-sulfatide Ab) and other PN.
Our results suggest that testing IgM anti-GM1 or anti-sulfatide Ab is useful only for diagnostic confirmation of specific subtypes of autoimmune PN.
确定抗GM1和抗硫脂抗体（Ab）在自身免疫性多发性神经病（PN）总体以及特定亚型中的临界滴度和诊断准确性。
在本研究中，通过酶联免疫吸附测定法检测了84例PN患者、120例癫痫患者和93名健康对照者的血清中的自身抗体，并通过薄层色谱法对结果进行了确认。比较了不同临界值下阳性患者的频率，以确定临界滴度。通过受试者操作特征分析确定准确性。
IgM抗GM1抗体的临界滴度为1:2000，IgM抗硫脂抗体的临界滴度为1:4000，这是总体自身免疫性PN的临界滴度。IgM抗GM1和抗硫脂抗体在自身免疫性PN与其他PN之间的鉴别能力较低，但在运动神经病（针对抗GM1抗体）或IgM副蛋白血症中的PN或慢性疼痛性感觉轴索性PN（针对抗硫脂抗体）与其他PN之间具有良好的鉴别能力。
我们的结果表明，检测IgM抗GM1或抗硫脂抗体仅有助于确诊自身免疫性PN的特定亚型。
