Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Neurochem Res. 2011 Jan;36(1):58-66. doi: 10.1007/s11064-010-0263-7. Epub 2010 Sep 19.
Campylobacter jejuni (C. jejuni) is frequently associated with axonal Guillain-Barré syndrome (GBS). We reported that C. jejuni DNA-binding protein from starved cells (C-Dps) binds to and damages myelinated nerves in vivo. We studied the binding patterns of C-Dps to nervous tissues and its in vitro effects on neural cells. Immunohistochemically, C-Dps labeled the nodes of Ranvier, the outermost parts of internodal myelin and the basement membrane in the peripheral nerves, and neurons and myelin in the central nervous tissues. Its binding was blocked by sulfatide. C-Dps bound to the cell surfaces of nerve growth factor (NGF)-treated PC12 cells leading to dose-dependent LDH release, which was inhibited by either heat-denaturation of C-Dps or coincubation with an anti-C-Dps mAb. However, its binding to the surfaces of cultured NSC34 cells, S16 cells, or dorsal root ganglion cells, did not induce cytotoxicity. These findings suggest a possible involvement of C-Dps in C. jejuni-related GBS.
空肠弯曲菌（C. jejuni）常与轴索性格林-巴利综合征（GBS）有关。我们曾报道过，饥饿细胞来源的空肠弯曲菌 DNA 结合蛋白（C-Dps）可与有髓神经发生结合并造成损伤。本研究旨在观察 C-Dps 与神经组织的结合模式及其对神经细胞的体外作用。免疫组化结果显示，C-Dps 标记了神经纤维的郎飞结、神经节段髓鞘的最外层和周围神经的基膜，以及中枢神经系统的神经元和髓鞘。神经生长因子（NGF）处理的 PC12 细胞表面的 C-Dps 结合可被唾液酸神经节苷脂（sulfatide）阻断。C-Dps 与 NGF 处理后的 PC12 细胞结合，导致乳酸脱氢酶（LDH）释放呈剂量依赖性，该作用可被 C-Dps 热变性或与抗 C-Dps mAb 共孵育所抑制。然而，C-Dps 与培养的 NSC34 细胞、S16 细胞或背根神经节细胞表面的结合并不诱导细胞毒性。这些发现提示 C-Dps 可能参与了与空肠弯曲菌相关的 GBS 发病过程。
