Nagakawa O, Ogasawara M, Murata J, Fuse H, Saiki I
Department of Pathogenic Biochemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Int J Urol. 2001 Feb;8(2):65-70. doi: 10.1046/j.1442-2042.2001.00250.x.
A previous study by the same authors demonstrated that among various neuropeptides in the prostate, calcitonin gene-related peptide (CGRP) and gastrin-releasing peptide (GRP) increased the invasive capacity of PC-3 prostate cancer cells through enhancement of cell motility, while substance P (SP) inhibited the invasiveness through suppression of motile response.
The effect of 10 kinds of neuropeptides were investigated, including CGRP, GRP, SP, neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin (CT), leucine-enkephalin (L-ENK), methionine-enkephalin (M-ENK), glucagon and parathyroid hormone-related protein (PTH-rP), on the invasion of DU-145 prostate cancer cells through a reconstituted basement membrane (Matrigel) and the haptotactic migration of DU-145, TSU-pr1 and LNCaP prostate cancer cells using a Transwell cell culture chamber assay.
It was found that GRP, CGRP and PTH-rP increased the invasive capacity of tumor cells. In contrast, SP, VIP, CT, L-ENK, M-ENK, NPY and glucagon had no significant effect. These three neuropeptides also increased the haptotactic migration of tumor cells to fibronectin. In addition VIP, CGRP and GRP increased the haptotactic migration of LNCaP prostate cancer cells and GRP and PTH-rP increased the migration of TSU-pr1 cells.
The results indicated that some prostatic neuropeptides increased the invasive potential of prostate cancer cells partially through enhancement of cell motility.
同一组作者之前的一项研究表明，在前列腺中的各种神经肽中，降钙素基因相关肽（CGRP）和胃泌素释放肽（GRP）通过增强细胞运动能力提高了PC-3前列腺癌细胞的侵袭能力，而P物质（SP）则通过抑制运动反应抑制了侵袭性。
研究了10种神经肽，包括CGRP、GRP、SP、神经肽Y（NPY）、血管活性肠肽（VIP）、降钙素（CT）、亮氨酸脑啡肽（L-ENK）、甲硫氨酸脑啡肽（M-ENK）、胰高血糖素和甲状旁腺激素相关蛋白（PTH-rP）对DU-145前列腺癌细胞通过重组基底膜（基质胶）的侵袭以及使用Transwell细胞培养小室试验对DU-145、TSU-pr1和LNCaP前列腺癌细胞的趋触性迁移的影响。
发现GRP、CGRP和PTH-rP提高了肿瘤细胞的侵袭能力。相比之下，SP、VIP、CT、L-ENK、M-ENK、NPY和胰高血糖素没有显著影响。这三种神经肽还增加了肿瘤细胞对纤连蛋白的趋触性迁移。此外，VIP、CGRP和GRP增加了LNCaP前列腺癌细胞的趋触性迁移，GRP和PTH-rP增加了TSU-pr1细胞的迁移。
结果表明，一些前列腺神经肽部分通过增强细胞运动能力提高了前列腺癌细胞的侵袭潜能。
