Capon A, Souil E, Gauthier B, Sumian C, Bachelet M, Buys B, Polla B S, Mordon S
UPRES EA 2689 INSERM IFR 22, Pavillon Vancostenobel, University Hospital, 59037 Lille, France.
Lasers Surg Med. 2001;28(2):168-75. doi: 10.1002/lsm.1035.
This study aimed to evaluate a 815-nm diode-laser system to assist wound closure to accelerate and improve healing process.
STUDY DESIGN/MATERIALS AND METHODS: A total of 25 male hairless rats (mutant OFA Sprague-Dawley rats, IFFA-CREDO, L'Arbresle, France) with four dorsal skin incisions were used for the study. For each wound, the good apposition of the edges was obtained with buried absorbable suture. In the laser group, the laser beam was applied spot by spot through a transparent adhesive dressing along two incisions with the following parameters: 1.5 W; 3 seconds; spot diameter, 2 mm; fluence, 145 J/cm(2). Both control wounds were closed with conventional suture techniques. The duration of the closure procedure was noted for each group. Clinical examination, histologic study, and measurement of tensile strength were performed at 3, 7, 15, and 21 days after surgery. Determination of activation of heat shock protein 70 (Hsp70) through immunocytochemistry was performed at days 1 and 7.
LASC was 4 times faster to process than conventional suture: 1 minute 49 +/- 20.6 seconds vs. 7 minutes 26 +/- 62.2 seconds. In the laser group, healing was accelerated resulting in a more indiscernible scar than in the control groups. Histologic aspect was better with earlier continuous epidermis and dermis and a thinner resulting scar. Tensile strength was 30 to 58% greater than in control groups at 7 and 15 days (P < 0.001). Expression of Hsp70 was markedly induced in skin structures examined after laser exposure.
This study shows the ability of the 815-nm diode-laser system to assist wound closure leading to an acceleration and an improvement of wound healing with indiscernible resulting scar. The mechanisms of this phenomenon are still unclear but further investigations are in progress to attempt to explain them.
本研究旨在评估815纳米二极管激光系统辅助伤口闭合，以加速和改善愈合过程。
研究设计/材料与方法：总共25只雄性无毛大鼠（突变型OFA斯普拉格-道利大鼠，法国IFFA-CREDO公司，拉布勒），背部有四个皮肤切口，用于本研究。对于每个伤口，通过埋藏可吸收缝线使边缘良好对合。在激光组中，通过透明粘性敷料沿两条切口逐点施加激光束，参数如下：1.5瓦；3秒；光斑直径2毫米；能量密度145焦/平方厘米。两个对照伤口均采用传统缝合技术闭合。记录每组闭合过程的持续时间。术后3天、7天、15天和21天进行临床检查、组织学研究和抗张强度测量。术后1天和7天通过免疫细胞化学测定热休克蛋白70（Hsp70）的激活情况。
激光辅助伤口闭合（LASC）的操作速度比传统缝合快4倍：1分49秒±20.6秒对7分26秒±62.2秒。在激光组中，愈合加速，形成的瘢痕比对照组更不明显。组织学表现更好，表皮和真皮更早连续，形成的瘢痕更薄。在7天和15天时，抗张强度比对照组高30%至58%（P<0.001）。激光照射后，在检查的皮肤结构中明显诱导了Hsp70的表达。
本研究表明815纳米二极管激光系统能够辅助伤口闭合，从而加速伤口愈合并改善愈合情况，形成的瘢痕不明显。这种现象的机制仍不清楚，但正在进行进一步研究以试图解释它们。
