Kunkitti Panisara, Bergqvist Ann-Sofi, Sjunnesson Ylva, Axnér Eva
Division of Reproduction, Department of Clinical Sciences, P.O. Box 7054, Swedish University of Agricultural Sciences, SLU, SE-750 07 Uppsala, Sweden; Department of Surgery and Theriogenology, Khon Kaen University, Khon Kaen 40002, Thailand.
Division of Reproduction, Department of Clinical Sciences, P.O. Box 7054, Swedish University of Agricultural Sciences, SLU, SE-750 07 Uppsala, Sweden.
Anim Reprod Sci. 2015 Oct;161:64-74. doi: 10.1016/j.anireprosci.2015.08.005. Epub 2015 Aug 20.
The sperm maturation process that occurs in the epididymis is a necessary process for spermatozoa to acquire motility and the ability to undergo capacitation, which is an important key for fertilization. The aim of this study was to evaluate the ability of feline spermatozoa from different regions of the epididymis to undergo capacitation and acrosome reaction. Experiment I: epididymal spermatozoa from caput, corpus and cauda regions were placed in phosphate buffered saline (control medium) and in vitro fertilization medium (capacitating conditions). Sperm motility, motility patterns, plasma membrane integrity and tyrosine phosphorylation were evaluated at time 0 and 60min after incubation. Experiment II: spermatozoa were treated with 2μM of calcium ionophore (A23187) to induce the acrosome reaction and acrosome reaction was evaluated. The results showed a significant effect of region with a higher percentage of tyrosine phosphorylation in spermatozoa from the cauda than in the caput or corpus regions (P=0.0061; P=0.0088). Spermatozoa from corpus and cauda showed higher values in the majority of the measured motility parameters than spermatozoa from the caput (P<0.0001). Spermatozoa from all epididymal regions can undergo the acrosome reaction in vitro in response to induction by calcium ionophore with no difference between regions (P>0.05). Spermatozoa from all epididymal regions were able to undergo capacitation. Higher percentage of tyrosine phosphorylation in spermatozoa from the cauda reflect that they more easily underwent capacitation compared to spermatozoa from caput and corpus which required more time of incubation for capacitation. In conclusion feline epididymal spermatozoa from all regions can undergo capacitation and acrosome reaction in vitro and do not require incubation under capacitating conditions.
发生在附睾中的精子成熟过程是精子获得运动能力和获能能力的必要过程,而获能是受精的重要关键。本研究的目的是评估来自附睾不同区域的猫精子的获能和顶体反应能力。实验I:将来自附睾头、体和尾区域的附睾精子置于磷酸盐缓冲盐水(对照培养基)和体外受精培养基(获能条件)中。在孵育0分钟和60分钟时评估精子活力、运动模式、质膜完整性和酪氨酸磷酸化。实验II:用2μM钙离子载体(A23187)处理精子以诱导顶体反应,并评估顶体反应。结果显示区域有显著影响,附睾尾精子中酪氨酸磷酸化的百分比高于附睾头或体区域(P = 0.0061;P = 0.0088)。附睾体和尾的精子在大多数测量的活力参数上显示的值高于附睾头的精子(P < 0.0001)。来自所有附睾区域的精子在体外对钙离子载体诱导均能发生顶体反应,各区域之间无差异(P > 0.05)。来自所有附睾区域的精子都能够获能。附睾尾精子中较高百分比的酪氨酸磷酸化反映出与附睾头和体的精子相比,它们更容易获能,附睾头和体的精子获能需要更长的孵育时间。总之,来自所有区域的猫附睾精子在体外都能发生获能和顶体反应,并且不需要在获能条件下孵育。