Rodríguez S, Caprile T
Instituto de Histología y Patología, Universidad Austral de Chile, Casilla 567 Valdivia, Chile. srodrig2@.uach.cl
Microsc Res Tech. 2001 Mar 1;52(5):564-72. doi: 10.1002/1097-0029(20010301)52:5<564::AID-JEMT1041>3.0.CO;2-7.
Reissner's fiber (RF) extends along the cerebral aqueduct, fourth ventricle, and the entire length of the central canal of the spinal cord. It grows continuously in the caudal direction by addition of newly released glycoproteins by the subcommissural organ (SCO) to its proximal end. Several hypotheses about RF function have been advanced. One of them postulates that RF binds biogenic amines present in the CSF and clears them away. In recent years, this hypothesis has been tested in our laboratory by using several experimental protocols. Firstly, the CSF concentration of monoamines was investigated in RF-deprived rats subjected to immunological neutralization of the SCO-RF complex. Secondly, the capacity of RF to bind monoamines in vivo was studied by injecting radiolabeled serotonin or noradrenaline into the rat CSF, and by perfusing them into the CSF, during one week, using an Alzet's osmotic pump. In vitro binding studies were performed using isolated bovine RF. All the findings obtained indicate that RF binds monoamines present in the ventricular CSF and then transports them along the central canal. In the absence of RF, the CSF concentration of monoamines increases sharply.
赖氏纤维（RF）沿中脑导水管、第四脑室及脊髓中央管全长延伸。它通过室管膜下器官（SCO）向其近端添加新释放的糖蛋白，从而不断向尾端生长。关于RF功能的几种假说已被提出。其中一种假说假定RF结合脑脊液中存在的生物胺并将其清除。近年来，我们实验室通过多种实验方案对这一假说进行了验证。首先，在对SCO-RF复合体进行免疫中和的RF缺失大鼠中，研究了单胺类物质在脑脊液中的浓度。其次，通过向大鼠脑脊液中注射放射性标记的5-羟色胺或去甲肾上腺素，并使用Alzet渗透泵在一周内将其灌注到脑脊液中，研究了RF在体内结合单胺类物质的能力。使用分离的牛RF进行了体外结合研究。所有获得的结果表明，RF结合脑室脑脊液中存在的单胺类物质，然后沿中央管运输它们。在没有RF的情况下，单胺类物质在脑脊液中的浓度会急剧增加。
