Wu Z, Zhang G, Shao K, Long M, Wang H, Song G, Wang B, Cai S
College of Bioengineering, Key Lab for Biomechanics and Tissue Engineering under the Ministry of Education, Chongqing University, Chongqing 400044, China.
Zhonghua Gan Zang Bing Za Zhi. 2001 Feb;9(1):25-7.
To investigate the relevance of the rheological properties, i.e., viscoelasticities and adhesion to basement membrane components coated surface, of both hepatocytes and hepatocellular carcinoma (HCC) cells to the cytoskeleton structure.
Micropipette aspiration technique was adopted to measure viscoelastic coefficients and adhesion forces to 2 microg/ml collagen IV/1.25 microg/ml laminin coated surface of the cells. Two kinds of cytoskeleton perturbing agents, colchicine and cytochalasin D, were used to treat both HCC cells and hepatocytes and the effects of these treatments on cell viscoelastic coefficients and cell adhesion forces were investigated.
Upon treatment of cells with colchicine in a concentration range of 1 to 60 mg/L, the elastic coefficients, especially the first elastic coefficient K1, and adhesion forces of hepatocytes generally tended to increase or increased significantly while, in contrast, viscoelastic coefficients and adhesion forces of HCC cells decreased obviously. Upon treatment of cells with cytochalasin D in a concentration range of 0.25-5.00 mg/L, viscoelastic coefficients of both hepatocytes and HCC cells decreased uniformly, with a larger magnitude for the decrease in elastic coefficients and adhesion forces of HCC cells than for those of hepatocytes. Adhesion forces of hepatocytes and HCC cells onto collagen IV/laminin coated surface varied similarly as viscoelastic coefficients under the action of the cytoskeleton perturbing agents. A significant positive correlation existed between changes of HCC cell adhesion forces on collagen IV/laminin coated surfaces and those of cell elastic coefficients (P<0.01).
The effects of cochicine and cytochalasin D on rheological properties of HCC cells differed significantly either in ways or extents from those on rheological properties of hepatocytes. These results might reflect the difference in the state of cytoskeleton structure and function among these two kinds of cells.
研究肝细胞和肝细胞癌（HCC）细胞的流变学特性，即粘弹性和对包被有基底膜成分的表面的粘附力，与细胞骨架结构的相关性。
采用微吸管抽吸技术测量细胞的粘弹性系数以及对包被有2μg/mlⅣ型胶原/1.25μg/ml层粘连蛋白的表面的粘附力。使用两种细胞骨架干扰剂秋水仙碱和细胞松弛素D处理HCC细胞和肝细胞，并研究这些处理对细胞粘弹性系数和细胞粘附力的影响。
用浓度范围为1至60mg/L的秋水仙碱处理细胞时，肝细胞的弹性系数，尤其是第一弹性系数K1和粘附力通常趋于增加或显著增加，而相比之下，HCC细胞的粘弹性系数和粘附力明显降低。用浓度范围为0.25 - 5.00mg/L的细胞松弛素D处理细胞时，肝细胞和HCC细胞的粘弹性系数均均匀降低，HCC细胞弹性系数和粘附力的降低幅度大于肝细胞。在细胞骨架干扰剂作用下，肝细胞和HCC细胞对Ⅳ型胶原/层粘连蛋白包被表面的粘附力变化与粘弹性系数相似。HCC细胞在Ⅳ型胶原/层粘连蛋白包被表面的粘附力变化与细胞弹性系数变化之间存在显著正相关（P<0.01）。
秋水仙碱和细胞松弛素D对HCC细胞流变学特性的影响在方式或程度上与对肝细胞流变学特性的影响显著不同。这些结果可能反映了这两种细胞在细胞骨架结构和功能状态上的差异。
