Sasaki M, Takahashi T, Miyahara K
Seikagaku Corporation, Central Research Laboratories, Higashiyamato City, Tokyo, Japan.
Spine (Phila Pa 1976). 2001 Mar 1;26(5):463-8. doi: 10.1097/00007632-200103010-00008.
In vivo intradiscal measurements of pressure in lumbar discs treated with chondroitinase ABC were performed.
To determine the decrease in lumbar intradiscal pressure after chemonucleolysis by chondroitinase ABC in sheep.
No previous study has assessed in vivo intradiscal pressure after chemonucleolysis. This study investigated the effect of chondroitinase ABC on intradiscal pressure in terms of a dose and time relation. It also included roentgenographic observations and evaluation of the correlation between disc space narrowing and decrease in intradiscal pressure.
Chondroitinase ABC was injected in the lumbar intervertebral discs of sheep at doses of 1, 5, and 50 U. Phosphate buffered saline also was injected as a negative control measure. One week before injection, then 1 and 4 weeks afterward, intradiscal pressure was measured using a catheter microtip pressure transducer. Simultaneously, standard lateral roentgenographs were taken, and the disc height index was calculated.
The intradiscal pressure clearly was decreased 1 week after chondroitinase ABC injection. A further decrease was observed up to 4 weeks. This pressure decrease was dose dependent. The disc height indexes also decreased with time, but the state of the change was different from that of the changes in intradiscal pressure. No clear quantitative correlation was found between intradiscal pressure and disc height index.
Chondroitinase ABC can induce the reduction of intradiscal pressure in the lumbar spine.
对用软骨素酶ABC治疗的腰椎间盘进行体内椎间盘内压力测量。
确定绵羊经软骨素酶ABC化学髓核溶解术后腰椎间盘内压力的降低情况。
以往尚无研究评估化学髓核溶解术后的体内椎间盘内压力。本研究从剂量和时间关系方面研究了软骨素酶ABC对椎间盘内压力的影响。还包括X线影像学观察以及椎间盘间隙变窄与椎间盘内压力降低之间相关性的评估。
将软骨素酶ABC以1、5和50单位的剂量注射到绵羊的腰椎间盘内。还注射磷酸盐缓冲盐水作为阴性对照措施。在注射前1周，然后在注射后1周和4周，使用导管微尖端压力传感器测量椎间盘内压力。同时，拍摄标准侧位X线片，并计算椎间盘高度指数。
软骨素酶ABC注射后1周，椎间盘内压力明显降低。直至4周观察到进一步降低。这种压力降低是剂量依赖性的。椎间盘高度指数也随时间降低，但变化状态与椎间盘内压力变化不同。在椎间盘内压力与椎间盘高度指数之间未发现明确的定量相关性。
软骨素酶ABC可诱导腰椎间盘内压力降低。
