Eurell J A, Brown M D, Ramos M
Department of Veterinary Biosciences, University of Illinois, Urbana 61801.
Clin Orthop Relat Res. 1990 Jul(256):238-43.
A series of intradiscal injections of chondroitinase ABC was performed in 31 young adult rabbits. Roentgenograms were taken immediately postinjection and were repeated at two, three, five, six, seven, or nine days after injection. All had roentgenographic evidence of disc space narrowing at all stages after the injection. Histologic evaluation of safranin-O fast green-stained sections indicated degradation of proteoglycans in the annulus fibrosus of chondroitinase ABC-injected discs. The cells of the chondroitinase ABC-injected discs, the end plates, and the growth-plate areas of adjacent vertebral bodies appeared unaffected.
对31只成年幼兔进行了一系列椎间盘内注射软骨素酶ABC的操作。注射后立即拍摄X线片，并在注射后2天、3天、5天、6天、7天或9天重复拍摄。所有兔子在注射后的各个阶段均有椎间盘间隙变窄的X线证据。对番红O固绿染色切片的组织学评估表明，注射软骨素酶ABC的椎间盘纤维环中的蛋白聚糖发生了降解。注射软骨素酶ABC的椎间盘细胞、终板以及相邻椎体的生长板区域似乎未受影响。
