Choi J Y, Kim J S, Lee J M, Hyun B H, Kim H S
Division of Biological Sciences, College of Natural Sciences, Pusan National University, Pusan 609-735, South Korea.
AIDS Res Hum Retroviruses. 2001 Mar 1;17(4):367-70. doi: 10.1089/08892220150503735.
A new human endogenous retroviral family (HERV-F) has been identified from human chromosome 7q31.1-q31.3 that was identical to the XA34 cDNA clone isolated from a human glioma cDNA library with an ERV-9 env probe. We investigated pol gene sequences of the HERV-F family from a human monochromosomal DNA panel and analyzed these with HERV-F. The pol gene sequences of the HERV-F family were detected on chromosomes 3, 6, 7, 10, 11, 14, 19, 20, X, and Y as examined by PCR. Thirty-six pol gene sequences identified from the human chromosomes have a high degree of sequence similarity (80-99%) with that of the HERV-F. Phylogenetic analysis of pol gene sequences distinctively showed four groups, indicating that the HERV-F family could be amplified at least four times after the original integration into the human genome or represent integration events separately during hominid evolution. One clone (HFY-3) on chromosome Y shared 100% sequence identity with a clone (HF19-2) on chromosome 19, and a clone (HF20-6) on chromosome 20 suggests either a recent retrotransposition or a chromosomal translocation. The history of endogenous retroviral sequences may contribute to an understanding of evolutionary change in human genomes.
一个新的人类内源性逆转录病毒家族（HERV-F）已从人类7号染色体q31.1-q31.3区域被鉴定出来，它与用ERV-9 env探针从人类胶质瘤cDNA文库中分离出的XA34 cDNA克隆相同。我们从人类单染色体DNA面板中研究了HERV-F家族的pol基因序列，并与HERV-F进行了分析。通过PCR检测发现，HERV-F家族的pol基因序列存在于3号、6号、7号、10号、11号、14号、19号、20号、X染色体和Y染色体上。从人类染色体中鉴定出的36个pol基因序列与HERV-F的序列具有高度的相似性（80-99%）。pol基因序列的系统发育分析明显显示出四个组，这表明HERV-F家族在最初整合到人类基因组后至少扩增了四次，或者在人类进化过程中分别代表了整合事件。Y染色体上的一个克隆（HFY-3）与19号染色体上的一个克隆（HF19-2）具有100%的序列同一性，20号染色体上的一个克隆（HF20-6）表明可能是最近的逆转座或染色体易位。内源性逆转录病毒序列的历史可能有助于理解人类基因组中的进化变化。
