Wade-Evans A M, Russell J, Jenkins A, Javan C
National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts, EN6 3QG UK.
AIDS Res Hum Retroviruses. 2001 Mar 1;17(4):371-5. doi: 10.1089/08892220150503744.
The characterization of several seven-transmembrane G protein-coupled receptors, which function as coreceptors for HIV-1, HIV-2, and/or SIV, has opened up a whole new area of AIDS research. Animal models that have played a central role in the understanding of lentivirus pathogenesis and the design of novel vaccine strategies may also be invaluable in studying the role of these secondary receptors in infection and disease progression. However, since it is known that minor species-specific sequence changes in CCR3 and STRL33 affect their ability to act as coreceptors for HIV-1, HIV-2, and/or SIV, it is important to ascertain whether the relevant receptors function as expected in the animal model of choice. Many studies have been performed on the function of rhesus macaque receptors, but not on the cynomolgus macaque equivalents. Both species are used as animal models for lentivirus pathogenesis, but since there are differences in their susceptibility to viral infection, we felt it was important for information to be available for both rhesus and cynomolgus macaque receptors. The sequence of three cynomolgus macaque receptors, CCR3, GPR15, and STRL33, are presented in this sequence note. These sequences are compared with already published human and rhesus macaque homologs. Functional studies are currently being performed on these three cynomolgus macaque receptors to determine their ability to function as coreceptors for HIV-2, SIV, and/or SHIV isolates.
几种作为HIV-1、HIV-2和/或SIV共受体的七跨膜G蛋白偶联受体的特性研究，开辟了艾滋病研究的全新领域。在理解慢病毒发病机制和设计新型疫苗策略方面发挥核心作用的动物模型，在研究这些辅助受体在感染和疾病进展中的作用时可能也具有重要价值。然而，由于已知CCR3和STRL33中微小的物种特异性序列变化会影响它们作为HIV-1、HIV-2和/或SIV共受体的能力，因此确定相关受体在所选动物模型中是否按预期发挥功能很重要。已经对恒河猴受体的功能进行了许多研究，但对食蟹猴的等效受体尚未进行研究。这两个物种都被用作慢病毒发病机制的动物模型，但由于它们对病毒感染的易感性存在差异，我们认为获得恒河猴和食蟹猴受体的信息都很重要。本序列注释中给出了三种食蟹猴受体CCR3、GPR15和STRL33的序列。这些序列与已发表的人类和恒河猴同源物进行了比较。目前正在对这三种食蟹猴受体进行功能研究，以确定它们作为HIV-2、SIV和/或SHIV分离株共受体的功能能力。
