Hyatt D R, Galland J C, Gillespie J R
Food Animal Health and Management Center, College of Veterinary Medicine, Kansas State University, Manhattan 66502, USA.
J Vet Diagn Invest. 2001 Jan;13(1):71-3. doi: 10.1177/104063870101300114.
The performance of a commercially available enzyme immunoassay (EIA) for determining the presence of Shiga toxin I and II in human diarrheal stool samples was evaluated for use as a presumptive test for the presence of Escherichia coli O157:H7 in nondiarrheal bovine fecal samples collected from 10 Kansas cow-calf ranches. The prevalence of E. coli O157:H7 in 2,297 samples, as determined by selective bacterial culture, was 1.6%. The sample prevalence of non-E. coli O157:H7 Shiga toxin-producing bacteria, as detected by the Shiga toxin EIA, was 5.8%. Only 2 of 136 samples that tested positive with the Shiga toxin EIA were positive for E. coli O157:H7 by culture. Compared with bacterial culture, the sensitivity of the Shiga toxin EIA was 5.5% and the specificity was 94.1%. Agreement between the 2 tests, as measured by the kappa statistic, was poor (kappa = -0.002). Although the Shiga toxin EIA was not a good presumptive test for the determination of E. coli O157:H7 in bovine fecal samples because of its low sensitivity (5.5%), it might be a useful test for the detection of Shiga toxin producing non-E. coli O157:H7 organisms in bovine feces.
评估了一种市售酶免疫测定法（EIA）在检测人类腹泻粪便样本中志贺毒素I和II的性能，以用作从堪萨斯州10个肉牛繁殖牧场采集的非腹泻牛粪便样本中是否存在大肠杆菌O157:H7的初步检测方法。通过选择性细菌培养测定，2297份样本中大肠杆菌O157:H7的流行率为1.6%。通过志贺毒素EIA检测，非大肠杆菌O157:H7产志贺毒素细菌的样本流行率为5.8%。在136份志贺毒素EIA检测呈阳性的样本中，只有2份经培养检测出大肠杆菌O157:H7呈阳性。与细菌培养相比，志贺毒素EIA的灵敏度为5.5%，特异性为94.1%。通过kappa统计量衡量，两种检测方法之间的一致性较差（kappa = -0.002）。尽管由于灵敏度较低（5.5%），志贺毒素EIA并非检测牛粪便样本中大肠杆菌O157:H7的良好初步检测方法，但它可能是检测牛粪便中产志贺毒素的非大肠杆菌O157:H7微生物的有用检测方法。
