Kitano S, Irimura K, Sasaki T, Abe N, Baba A, Miyake Y, Katunuma N, Yamamoto K
Pharmacology Research Laboratory, Tokushima Research Center, Taiho Pharmaceutical Co., Ltd., Japan.
Jpn J Pharmacol. 2001 Jan;85(1):84-91. doi: 10.1254/jjp.85.84.
In this study, we developed a procedure to produce gingivitis in rats by inoculation of Porphyromonas gingivalis and studied the contribution of the bacterial cysteine proteinases, Arg-gingipain (Rgp) and Lys-gingipain (Kgp), to the pathology in the gingiva. To adhere the bacterium to periodontal tissues, a cotton thread was inserted between the first and second molar of right maxillary sites of rats. Rats in group A were administered with vehicle alone after bacterial (strain W83) inoculation. In group B, the bacteria were inoculated in combination with leupeptin, a potent inhibitor of Rgp and Kgp, and then leupeptin alone was administered the week after. Rats in group C were administered leupeptin for 6 weeks after bacteria inoculation. All left maxillary gingiva in three groups showed no inflammatory changes. Right maxillary gingiva of group A showed most of the clinical landmarks of gingivitis. Leupeptin exhibited only a little inhibitory effect on this gingivitis in group B, whereas it had a strong inhibitory effect on the inflammation in group C. These results suggest that P. gingivalis-induced gingivitis is attributable to Rgp and Kgp and that leupeptin is more effective in the late phase than the early stage of gingivitis.
在本研究中，我们开发了一种通过接种牙龈卟啉单胞菌在大鼠中诱发牙龈炎的方法，并研究了细菌半胱氨酸蛋白酶精氨酸牙龈蛋白酶（Rgp）和赖氨酸牙龈蛋白酶（Kgp）对牙龈组织病理学的影响。为使细菌黏附于牙周组织，将一根棉线插入大鼠右上颌第一和第二磨牙之间。A组大鼠在接种细菌（W83菌株）后仅给予赋形剂。B组在接种细菌时联合给予亮抑酶肽（一种Rgp和Kgp的强效抑制剂），然后在一周后单独给予亮抑酶肽。C组大鼠在接种细菌后给予亮抑酶肽6周。三组大鼠的左侧上颌牙龈均未出现炎症变化。A组右侧上颌牙龈表现出牙龈炎的大部分临床特征。亮抑酶肽对B组的这种牙龈炎仅表现出轻微的抑制作用，而对C组的炎症有强烈的抑制作用。这些结果表明，牙龈卟啉单胞菌诱发的牙龈炎归因于Rgp和Kgp，并且亮抑酶肽在牙龈炎后期比早期更有效。
