Cao A, Balant L, Fabre J, Canavèse J C
Schweiz Med Wochenschr. 1975 Apr 5;105(14):421-9.
Since detection of the first stage of nephropathies requires an exact definition of physiological proteinuria, this has been sought in 97 healthy individuals. Measured by a modification of the biuret reaction, physiological proteinuria did not exceed 100 mug/min in the recumbent position (average of 24.5 mug/min) and 150 mg/24 h (average of 51 mg/24 h). No significant differences were seen with respect to age or sex. Qualitative analysis of urinary proteins was done by cellulose acetate electrophoresis, immunoelectrophoresis and polyacrylamide-SDS electrophoresis. This latter method has the great advantage of classifying the proteins according to their molecular weight, wtihout electrostatic interference. Its graphic representation delineates a normal zone allowing an objective distinction between physiological and pathological proteinurias. In the majority of cases, the proportion of albumin is between 25 and 55%. The orthostatic position increases proteinuria (average of 41 mug/min) with a tendency to a distribution which erroneously suggests a glomerulopathy. Accordingly, investigation for small changes in proteinuria should always be carried out on urine formed in the recumbent position.
由于检测肾病的第一阶段需要对生理性蛋白尿进行精确界定，因此对97名健康个体进行了此项研究。通过改良的双缩脲反应进行测量，生理性蛋白尿在卧位时不超过100微克/分钟（平均为24.5微克/分钟），24小时不超过150毫克（平均为51毫克/24小时）。在年龄和性别方面未观察到显著差异。通过醋酸纤维素电泳、免疫电泳和聚丙烯酰胺-SDS电泳对尿蛋白进行定性分析。后一种方法具有很大的优势，即可以根据蛋白质的分子量对其进行分类，而不受静电干扰。其图形表示描绘了一个正常区域，从而能够客观地区分生理性和病理性蛋白尿。在大多数情况下，白蛋白的比例在25%至55%之间。直立位会增加蛋白尿（平均为41微克/分钟），其分布趋势错误地提示可能存在肾小球病。因此，对蛋白尿微小变化的调查应始终针对卧位时形成的尿液进行。
