Gao Z, Huang K, Xu H
Department of Chemistry, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.
Pharmacol Res. 2001 Feb;43(2):173-8. doi: 10.1006/phrs.2000.0761.
Oxidative stress plays an important role in the pathological process of neurodegenerative diseases. The effects of four major flavonoids present in Scutellaria baicalensis Georgi on hydrogen peroxide-induced neuronal cell damage are studied in this paper. When human neuroblastoma SH-SY5Y cells were incubated in Hanks' solution with the addition of 400 microM hydrogen peroxide for 2 h, the viability of cells was decreased remarkably, while the cell lipid peroxidation and the percentage of lactose dehydrogenase released into the culture medium was significantly increased. Addition of 10 microM of baicalein and baicalin significantly attenuated the cellular injury induced by hydrogen peroxide, while the effect of wogonin was marginal and wogonoside showed no effect at the tested concentration. In a separate experiment, 10 microM of baicalein and baicalin also antagonized the intracellular free-calcium concentration ([Ca2+]i) increase caused by 1 mM hydrogen peroxide. The effects of baicalein in both experiments were similar to those of quercetin, a well-studied antioxidant flavonoid. These results demonstrated the protective effects of flavonoids originating from Scutellaria baicalensis Georgi on the oxidative injury of neuronal cells.
氧化应激在神经退行性疾病的病理过程中起着重要作用。本文研究了黄芩中四种主要黄酮类化合物对过氧化氢诱导的神经元细胞损伤的影响。当人神经母细胞瘤SH-SY5Y细胞在含有400微摩尔过氧化氢的汉克斯溶液中孵育2小时时，细胞活力显著下降，而细胞脂质过氧化和释放到培养基中的乳酸脱氢酶百分比显著增加。添加10微摩尔的黄芩素和黄芩苷可显著减轻过氧化氢诱导的细胞损伤，而汉黄芩素的作用微弱，汉黄芩苷在测试浓度下无作用。在另一个实验中，10微摩尔的黄芩素和黄芩苷也拮抗了1毫摩尔过氧化氢引起的细胞内游离钙浓度（[Ca2+]i）升高。在两个实验中，黄芩素的作用与槲皮素（一种研究充分的抗氧化黄酮类化合物）的作用相似。这些结果证明了源自黄芩的黄酮类化合物对神经元细胞氧化损伤的保护作用。
