Guh J Y, Chen H C, Chuang L Y, Yang C Y, Tsai J H, Lai Y H
Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
Nephron. 2001 Feb;87(2):134-8. doi: 10.1159/000045901.
BACKGROUND/AIMS: Hemodialysis (HD) patients are prone to developing peptic ulcers. However, of all the risk factors associated with peptic ulcers, none have been shown to be more prevalent in HD patients than in the general population. However, salivary epidermal growth factor (EGF) may play a role in peptic ulcer diseases.
Salivary EGF levels and bioactivities were assayed in 47 maintenance HD patients and 30 normal controls, and the molecular weights of EGF were assessed using high-performance liquid chromatography (HPLC).
Salivary EGF levels were not different between both groups of subjects (4.2 +/- 0.34 vs. 5 +/- 0.54 ng/mg protein, NS), and HPLC revealed that salivary EGF in both groups had similar molecular weights. However, salivary EGF bioactivity was significantly depressed in the HD patients as compared to the normal controls (0.59 +/- 0.08 vs. 1.55 +/- 0.15 ng/mg protein, p < 0.01). Stepwise multiple regression showed that the low salivary EGF levels were associated with female gender (p < 0.05), while low salivary EGF bioactivity was associated with HD per se (p < 0.05). In the 22 HD patients who underwent gastric endoscopy, salivary EGF bioactivity was significantly lower in those with peptic ulcers than in those without (0.38 +/- 0.08 vs. 0.69 +/- 0.08 ng/mg protein, p < 0.05).
Decreased salivary EGF bioactivity may contribute to peptic ulcer disease among maintenance HD patients.
背景/目的：血液透析（HD）患者容易发生消化性溃疡。然而，在所有与消化性溃疡相关的危险因素中，没有一种在HD患者中比在普通人群中更普遍。然而，唾液表皮生长因子（EGF）可能在消化性溃疡疾病中起作用。
检测了47例维持性HD患者和30例正常对照者的唾液EGF水平和生物活性，并使用高效液相色谱（HPLC）评估EGF的分子量。
两组受试者的唾液EGF水平无差异（4.2±0.34对5±0.54 ng/mg蛋白质，无显著性差异），HPLC显示两组的唾液EGF分子量相似。然而，与正常对照相比，HD患者的唾液EGF生物活性显著降低（0.59±0.08对1.55±0.15 ng/mg蛋白质，p<0.01）。逐步多元回归显示，唾液EGF水平低与女性性别有关（p<0.05），而唾液EGF生物活性低与HD本身有关（p<0.05）。在22例接受胃镜检查的HD患者中，有消化性溃疡的患者唾液EGF生物活性显著低于无消化性溃疡的患者（0.38±0.08对0.69±0.08 ng/mg蛋白质，p<0.05）。
唾液EGF生物活性降低可能是维持性HD患者消化性溃疡疾病的一个原因。
