Deng F, Li G, Yand G H
Department of Pathology, West China University of Medical Sciences, Chengdo 610041.
Zhonghua Bing Li Xue Za Zhi. 1998 Aug;27(4):265-8.
To investigate the origin and cell lineage of Hodgkin/Reed-Sternberg (H/R-S) cells from various subtypes of Hodgkin's disease (HD).
68 single H/R-S cells were taken from frozen section of 8 cases of HD. The DNA from these single cells was amplified by polymerase chain reaction (PCR) with immunoglobulin heavy chain gene FR III a/JH primers and light chain family-specific primers.
(1) IgH and V kappa 4 rearrangements were repeatedly found in H/R-S cells from one case of lymphocytic predominant HD (LPHD). (2) Repeated and unique IgH and V kappa 2,4 rearrangements were observed in 1 case of nodular sclerosing HD (NSHD) and unique IgH, V lambda 3/V kappa 4 rearrangements in another case of NSHD. (3) Repeated IgH/V lambda 3 and unique V lambda 2,4 rearrangements, repeated V kappa 4 and unique IgH/V kappa 3 rearrangements, plus repeated IgH and unique V kappa 3/V lambda 4 rearrangements were detected respectively in 3 cases of mixed cellularity HD (MCHD). Repeated and unique rearrangements were found respectively in 2 cases of MCHD.
The H/R-S cells isolated from LPHD had monoclonal Igh and V kappa 4 gene rearrangements, which supports the conclusion that LPHD is a monoclonal proliferation of B cells. The appearance of IgH and kappa, lambda gene rearrangement suggest that the H/R-S cells isolated from classical HD (MCHD and NSHD) originate from B lineage cells at various stages of differentiation.
研究霍奇金淋巴瘤（HD）各亚型中霍奇金/里德-斯腾伯格（H/R-S）细胞的起源和细胞谱系。
从8例HD的冰冻切片中获取68个单个H/R-S细胞。使用免疫球蛋白重链基因FR III a/JH引物和轻链家族特异性引物，通过聚合酶链反应（PCR）扩增这些单个细胞的DNA。
（1）在1例淋巴细胞为主型HD（LPHD）的H/R-S细胞中反复发现IgH和Vκ4重排。（2）在1例结节硬化型HD（NSHD）中观察到反复且独特的IgH和Vκ2、4重排，在另一例NSHD中观察到独特的IgH、Vλ3/Vκ4重排。（3）在3例混合细胞型HD（MCHD）中分别检测到反复的IgH/Vλ3和独特的Vλ2、4重排，反复的Vκ4和独特的IgH/Vκ3重排，以及反复的IgH和独特的Vκ3/Vλ4重排。在2例MCHD中分别发现了反复和独特的重排。
从LPHD分离出的H/R-S细胞具有单克隆Igh和Vκ4基因重排，这支持LPHD是B细胞单克隆增殖的结论。IgH和κ、λ基因重排的出现表明，从经典HD（MCHD和NSHD）分离出的H/R-S细胞起源于不同分化阶段的B谱系细胞。
