Orazi A, Jiang B, Lee C H, English G W, Cattoretti G, John K, Neiman R S
Division of Hematopathology, Indiana University School of Medicine, Indianapolis, USA.
Am J Clin Pathol. 1995 Oct;104(4):413-8. doi: 10.1093/ajcp/104.4.413.
Southern blot analysis of Hodgkin's disease (HD), although often compromised by the small number of abnormal cells present in the tissue, have tended to favor a B-cell derivation of the Hodgkin's and Reed-Sternberg (HRS) cells in cases of nodular sclerosis (NS) and mixed cellularity (MC) Hodgkin's disease. Eighteen frozen and 29 paraffin-embedded sections of lymph node specimens from 29 patients with pretreatment HD (22 NSHD and 7 MCHD) were studied by molecular analysis and immunohistochemistry to determine the phenotype of HRS cells. All cases were reviewed and showed typical morphology and CD45-, CD30+, CD15+, BLA.36+ HRS cells. In 11 of 29 (38%) cases, HRS cells were reactive with at least one B-cell marker (CD20, CD79a, MB2), 7 of 29 (24%) cases showed reactivity with the T-cell marker CD3, and 11 of 29 (38%) cases displayed a "null" phenotype. By using a polymerase chain reaction (PCR) and consensus primers for the V and J regions of the immunoglobulin heavy chain (IgH) gene, the authors were able to detect B-cell clonality in 9 of 18 (50%) frozen samples of HD analyzed. IgH gene rearrangement was present in 8 of 15 (53%) NSHD and in 1 of 3 (33%) MCHD. In five of nine (56%) of these cases, HRS cells were reactive with at least one B-cell marker, whereas one case expressed the T-cell marker CD3. The other three cases with IgH gene rearrangement showed a "null" immunophenotype. IgH gene analysis was negative in all remaining CD3+ cases and in two other cases that expressed B-cell markers by immunohistology. Southern blotting failed to detect rearrangement of the T-cell receptor beta-chain gene and immunoglobulin heavy and light genes in any of these cases. The results show that PCR represents a specific and sensitive technique for the detection of IgH gene rearrangements in cases of Hodgkin's disease. The results also suggest a lymphoid B-cell derivation of HRS cells in a high proportion of the cases.
霍奇金淋巴瘤（HD）的Southern印迹分析，尽管常常因组织中存在的异常细胞数量少而受到影响，但在结节硬化型（NS）和混合细胞型（MC）霍奇金淋巴瘤病例中，倾向于支持霍奇金和里德-斯腾伯格（HRS）细胞来源于B细胞。通过分子分析和免疫组织化学研究了29例HD患者（22例NSHD和7例MCHD）预处理前淋巴结标本的18份冰冻切片和29份石蜡包埋切片，以确定HRS细胞的表型。所有病例均经复查，显示典型形态以及CD45阴性、CD30阳性、CD15阳性、BLA.36阳性的HRS细胞。在29例（38%）病例中，11例HRS细胞与至少一种B细胞标志物（CD20、CD79a、MB2）反应，29例（24%）病例中的7例与T细胞标志物CD3反应，29例（38%）病例表现为“无”表型。通过使用针对免疫球蛋白重链（IgH）基因V和J区域的聚合酶链反应（PCR）和共有引物，作者在分析的18份HD冰冻样本中的9份（50%）中检测到了B细胞克隆性。IgH基因重排在15例NSHD中的8例（53%）和3例MCHD中的1例（33%）中存在。在这些病例中的9例（56%）中的5例中，HRS细胞与至少一种B细胞标志物反应，而1例表达T细胞标志物CD3。其他3例IgH基因重排的病例表现为“无”免疫表型。在所有其余CD3阳性病例以及另外2例通过免疫组织化学表达B细胞标志物的病例中，IgH基因分析均为阴性。在所有这些病例中，Southern印迹均未检测到T细胞受体β链基因以及免疫球蛋白重链和轻链基因的重排。结果表明，PCR是检测霍奇金淋巴瘤病例中IgH基因重排的一种特异性和敏感性技术。结果还提示，在高比例病例中HRS细胞来源于淋巴样B细胞。
