层粘连蛋白受体抗体与蛋白激酶C抑制剂体内抑制肿瘤转移能力的实验研究

[An experimental study on the capability of laminin receptor antibody and protein kinase C inhibitor to inhibit tumor metastasis in vivo].

作者信息

Xu T, SiTu R

机构信息

Department of Pathology, Jinan University Medical College, Guangzhou 510632.

出版信息

Zhonghua Bing Li Xue Za Zhi. 1998 Oct;27(5):344-7.

PMID:11245008

Abstract

OBJECTIVE

To study methods for blocking tumor metastasis.

METHODS

Endogenous laminin (LN), protein kinase C (PKC) inhibitor staurosporine (SP), anti-LN receptor (LN-R) were used to act on murine fibrosarcoma cells which were strongly positive for endogenous LN and LN-R, then infused into the caudal veins of 77 C57 mice once, to initiate lung tumor formation. Before infusion, the cells were all measured for surface LN-R quantity with flow cytometry.

RESULTS

The positive rate of LN-R on tumor cell surface in the control group, LN group, SP group, anti-LN-R group and anti-LN group being 56.5%, 76.3%, 50.2%, 31.9% and 20.6% respectively. The average volume of the tumors in the lungs of mice being 76 mm3, 110 mm3, 14 mm3, 5 mm3 and 20 mm3 respectively. The rate of tumor embolus formation in lymphatic and blood vessels being 81%, 94%, 36%, 43% and 68% respectively.

CONCLUSION

The pathological changes and metastasis in the LN group are more svere than that in control group. But all other groups are less severe than the control group. The level of LN-R on tumor cell surface is positively correlated with tumor volume and rat of tumor embolus formation. The lipositol conduction system in tumor cells is also closely related with size of tumor foci and rate of tumor embolus formation.

摘要

目的

研究阻断肿瘤转移的方法。

方法

用内源性层粘连蛋白（LN）、蛋白激酶C（PKC）抑制剂星形孢菌素（SP）、抗LN受体（LN-R）作用于内源性LN和LN-R呈强阳性的小鼠纤维肉瘤细胞，然后一次性注入77只C57小鼠的尾静脉，以引发肺部肿瘤形成。在注入前，用流式细胞仪检测所有细胞表面的LN-R数量。

结果

对照组、LN组、SP组、抗LN-R组和抗LN组肿瘤细胞表面LN-R的阳性率分别为56.5%、76.3%、50.2%、31.9%和20.6%。小鼠肺部肿瘤的平均体积分别为76立方毫米、110立方毫米、14立方毫米、5立方毫米和20立方毫米。淋巴管和血管中肿瘤栓子形成率分别为81%、94%、36%、43%和68%。