[Laminin distribution in the clonal cells of PLA-801 (pulmonary giant cell carcinoma) cell line and its effect on tumor cell migration].

LN distribution in clone C and clone D cells of the PLA-801 (pulmonary giant cell carcinoma) cell line was studied with immunocytochemical staining. There were less LN and LN receptors found in clone C cells, which were from a low metastatic cell line, but much more LN and LNR were obtained in the highly metastatic clone D cells. The in vitro migration rates of these 2 clones were measured with agarose migratory analysis. The rate of clone D was much greater than that of clone C. With the presence of exgenous LN, clone C cells became activated with a higher migration rate and the migration rate of clone D was greatly reduced when cells were pretreated with anti-LN antibody. These results indicated that LN and LNR may play a vital role in the migration and metastasis of tumor cells.