Prieur I, Lunardi J, Dupuis A
Laboratoire de Bioénergétique Cellulaire et Pathologique (EA 2943-UJF), Département de Biologie Moléculaire et Structurale CEA Grenoble, 17 rue des Martyrs, 38054 Cedex 9, Grenoble, France.
Biochim Biophys Acta. 2001 Apr 2;1504(2-3):173-8. doi: 10.1016/s0005-2728(01)00158-x.
Piericidin, rotenone and pyridaben are specific inhibitors of the NADH-ubiquinone oxidoreductase (Complex I) that bind to its ubiquinone binding site(s). Using site directed mutagenesis, we demonstrate that residues G409, D412, R413 and V407 of the C-terminus of Complex I NUOD subunit are directly involved in the binding of these inhibitors. We propose that the corresponding inhibitor/quinone binding site would be located close to NUOD-NUOB interface.
杀粉蝶菌素、鱼藤酮和哒螨灵是NADH-泛醌氧化还原酶（复合体I）的特异性抑制剂，它们与该酶的泛醌结合位点结合。利用定点诱变技术，我们证明了复合体I的NUOD亚基C末端的G409、D412、R413和V407残基直接参与这些抑制剂的结合。我们推测相应的抑制剂/泛醌结合位点可能位于NUOD-NUOB界面附近。
