• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Genetic evidence for the existence of two quinone related inhibitor binding sites in NADH-CoQ reductase.

作者信息

Darrouzet E, Dupuis A

机构信息

Département de Biologie Moléculaire et Structurale, CEA Grenoble, France.

出版信息

Biochim Biophys Acta. 1997 Mar 28;1319(1):1-4. doi: 10.1016/s0005-2728(97)00009-1.

DOI:10.1016/s0005-2728(97)00009-1
PMID:9107311
Abstract

Using the NADH-CoQ reductase of Rhodobacter capsulatus as a model for the mitochondrial Complex I, we have for the first time isolated bacterial mutants resistant to piericidin-A, a classical inhibitor of the mitochondrial enzyme. Their sensitivity to other inhibitors directed towards the quinone binding domain of complex I gives direct genetic evidence for the existence of two inhibitor binding sites.

摘要

相似文献

1
Genetic evidence for the existence of two quinone related inhibitor binding sites in NADH-CoQ reductase.
Biochim Biophys Acta. 1997 Mar 28;1319(1):1-4. doi: 10.1016/s0005-2728(97)00009-1.
2
Evidence for a quinone binding site close to the interface between NUOD and NUOB subunits of Complex I.有证据表明，醌结合位点靠近复合体I的NUOD和NUOB亚基之间的界面。
Biochim Biophys Acta. 2001 Apr 2;1504(2-3):173-8. doi: 10.1016/s0005-2728(01)00158-x.
3
The 49-kDa subunit of NADH-ubiquinone oxidoreductase (Complex I) is involved in the binding of piericidin and rotenone, two quinone-related inhibitors.NADH-泛醌氧化还原酶（复合体I）的49-kDa亚基参与了杀粉蝶菌素和鱼藤酮这两种醌类相关抑制剂的结合。
FEBS Lett. 1998 Jul 10;431(1):34-8. doi: 10.1016/s0014-5793(98)00719-4.
4
Natural substances (acetogenins) from the family Annonaceae are powerful inhibitors of mitochondrial NADH dehydrogenase (Complex I).番荔枝科的天然物质（乙酰精）是线粒体NADH脱氢酶（复合体I）的强效抑制剂。
Biochem J. 1994 Jul 1;301 ( Pt 1)(Pt 1):161-7. doi: 10.1042/bj3010161.
5
Evidence that the blockade of mitochondrial respiration by the neurotoxin 1-methyl-4-phenylpyridinium (MPP+) involves binding at the same site as the respiratory inhibitor, rotenone.神经毒素1-甲基-4-苯基吡啶离子（MPP+）对线粒体呼吸的阻断作用与呼吸抑制剂鱼藤酮在同一部位结合的证据。
Biochem Biophys Res Commun. 1990 May 31;169(1):123-8. doi: 10.1016/0006-291x(90)91442-u.
6
Evidence that the inhibition sites of the neurotoxic amine 1-methyl-4-phenylpyridinium (MPP+) and of the respiratory chain inhibitor piericidin A are the same.神经毒性胺1-甲基-4-苯基吡啶鎓（MPP+）和呼吸链抑制剂粉蝶霉素A的抑制位点相同的证据。
Biochem J. 1991 Jan 15;273(Pt 2)(Pt 2):481-4. doi: 10.1042/bj2730481.
7
NADPH-dependent coenzyme Q reductase is the main enzyme responsible for the reduction of non-mitochondrial CoQ in cells.NADPH 依赖性辅酶 Q 还原酶是负责细胞中非线粒体辅酶 Q 还原的主要酶。
Biofactors. 2008;32(1-4):59-70. doi: 10.1002/biof.5520320108.
8
Two EPR-detectable [4Fe-4S] clusters, N2a and N2b, are bound to the NuoI (TYKY) subunit of NADH:ubiquinone oxidoreductase (Complex I) from Rhodobacter capsulatus.两个可用电子顺磁共振检测到的[4铁-4硫]簇，即N2a和N2b，与来自荚膜红细菌的NADH：泛醌氧化还原酶（复合体I）的NuoI（TYKY）亚基结合。
Biochim Biophys Acta. 2003 Mar 6;1557(1-3):51-66. doi: 10.1016/s0005-2728(02)00398-5.
9
Relation of superoxide generation and lipid peroxidation to the inhibition of NADH-Q oxidoreductase by rotenone, piericidin A, and MPP+.超氧化物生成和脂质过氧化与鱼藤酮、杀粉蝶菌素A和MPP +对NADH-Q氧化还原酶抑制作用的关系。
Biochem Biophys Res Commun. 1992 Nov 30;189(1):47-52. doi: 10.1016/0006-291x(92)91523-s.
10
The second coenzyme Q1 binding site of bovine heart NADH: coenzyme Q oxidoreductase.
J Bioenerg Biomembr. 2002 Apr;34(2):89-94. doi: 10.1023/a:1015119808009.

引用本文的文献

1
Conditional essentiality of the 11-subunit complex I-like enzyme in strict anaerobes: the case of strain DCB-2.严格厌氧菌中11亚基类复合体I酶的条件必需性：以菌株DCB-2为例
Front Microbiol. 2024 Jun 26;15:1388961. doi: 10.3389/fmicb.2024.1388961. eCollection 2024.
2
Essential regions in the membrane domain of bacterial complex I (NDH-1): the machinery for proton translocation.细菌复合物I（NDH-1）膜结构域中的关键区域：质子转运机制
J Bioenerg Biomembr. 2014 Aug;46(4):279-87. doi: 10.1007/s10863-014-9558-8. Epub 2014 Jun 29.
3
Molecular characterization of benzimidazole resistance in Helicobacter pylori.
幽门螺杆菌对苯并咪唑耐药性的分子特征分析
Antimicrob Agents Chemother. 2004 Jul;48(7):2524-30. doi: 10.1128/AAC.48.7.2524-2530.2004.
4
EPR characterization of ubisemiquinones and iron-sulfur cluster N2, central components of the energy coupling in the NADH-ubiquinone oxidoreductase (complex I) in situ.原位对泛半醌和铁硫簇N2进行电子顺磁共振表征，它们是NADH-泛醌氧化还原酶（复合体I）中能量耦合的核心组分。
J Bioenerg Biomembr. 2002 Jun;34(3):193-208. doi: 10.1023/a:1016083419979.
5
The origin of cluster N2 of the energy-transducing NADH-quinone oxidoreductase: comparisons of phylogenetically related enzymes.能量转换型NADH-醌氧化还原酶N2簇的起源：系统发育相关酶的比较
J Bioenerg Biomembr. 2001 Jun;33(3):213-22. doi: 10.1023/a:1010782903144.
6
Toward a characterization of the connecting module of complex I.关于复合体I连接模块的特性研究
J Bioenerg Biomembr. 2001 Jun;33(3):159-68. doi: 10.1023/a:1010770600418.