Jemiota-Rzemińska M, Latowski D, Strzałka K
Department of Plant Physiology and Biochemistry, The Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Al. Mickiewicza 3, 31-120 Kraków, Poland.
Chem Phys Lipids. 2001 Mar;110(1):85-94. doi: 10.1016/s0009-3084(00)00227-9.
The efficiency of incorporation of plastoquinones and ubiquinones into phospholipid liposomes has been studied. The representatives of short (PQ1 and UQ1) middle (PQ4 and UQ4) and long (PQ9, UQ9 and UQ10) prenylquinones have been used to investigate the effect of quinone side chain length. The properties of hydroquinones have been also thoroughly examined in relation to the quinone forms. The extraction procedure was modified and further developed which enables removing of nonincorporated quinone by pentane washing and then determination of quinone content inside the lipid bilayer. The quantitatively evaluation of the amount of prenylquinone was assayed by means of HPLC analysis which offers much greater sensitivity and could be easily applied in case of hydroquinones. It has been found that PQ1 and UQ1 as well as their reduced forms were present mainly (about 80%) in the aqueous phase, when attempting to introduce them into phospholipid bilayer. In case of quinones having four and more isoprenyl units in side chain, a high level of quinone incorporation, ranging about 95%, was observed. The results pointed out that when comparing the effects of different exogenous quinones on membrane related processes, one has to consider the effectiveness of their incorporation within lipid bilayer.
已对质体醌和泛醌掺入磷脂脂质体的效率进行了研究。使用了短链（PQ1和UQ1）、中链（PQ4和UQ4）和长链（PQ9、UQ9和UQ10）异戊二烯醌的代表物来研究醌侧链长度的影响。还对氢醌的性质与醌形式的关系进行了深入研究。对提取程序进行了改进和进一步开发，通过戊烷洗涤去除未掺入的醌，然后测定脂质双层内的醌含量。通过HPLC分析对异戊二烯醌的量进行定量评估，该方法具有更高的灵敏度，并且在氢醌的情况下易于应用。已发现，当试图将PQ1和UQ1及其还原形式引入磷脂双层时，它们主要（约80%）存在于水相中。对于侧链具有四个及更多异戊二烯单元的醌，观察到约95%的高醌掺入水平。结果指出，在比较不同外源性醌对膜相关过程的影响时，必须考虑它们在脂质双层中的掺入效率。
