Stenvik J, Schrøder M B, Olsen K, Zapata A, Jørgensen T O
The Norwegian College of Fishery Science, Department of Marine Biotechnology, University of Tromsø, N-9037 Tromsø, Norway.
Dev Comp Immunol. 2001 May;25(4):291-302. doi: 10.1016/s0145-305x(00)00056-2.
Expression of the immunoglobulin (Ig) heavy chain transcripts in spleen and head kidney of the Atlantic cod (Gadus morhua L.) was investigated using in situ hybridization (ISH) and northern blotting. Specific detection of plasma cells was done with a probe for secretory IgM transcripts (mu 4). The plasma cells were often clustered close to blood vessels. Cells expressing surface IgM and IgD transcripts were detected using ISH with tyramide signal amplification (TSA). The positive cells were more abundant than plasma cells, had a lymphocyte-like morphology, and were evenly distributed throughout the tissues. This suggests that cod IgD mainly is expressed as a B-cell receptor akin to IgD in mammals. The VH-III family dominated the repertoire within the plasma cells, in agreement with data from cDNA cloning. Immunization with hapten-carrier antigen did not induce a systemic antibody response, and neither was any change in the clustering or distribution pattern of plasma cells within the tissues seen. A few clusters of plasma cells expressed only the rare VH-I and VH-II families, suggesting an ongoing clonal expansion and differentiation in these regions independently of immunization.
利用原位杂交（ISH）和Northern印迹法，研究了大西洋鳕鱼（Gadus morhua L.）脾脏和头肾中免疫球蛋白（Ig）重链转录本的表达情况。使用针对分泌型IgM转录本（μ4）的探针特异性检测浆细胞。浆细胞常常聚集在血管附近。使用酪胺信号放大（TSA）的原位杂交法检测表达表面IgM和IgD转录本的细胞。阳性细胞比浆细胞更丰富，具有淋巴细胞样形态，并且均匀分布于整个组织中。这表明鳕鱼IgD主要作为类似于哺乳动物IgD的B细胞受体表达。VH-III家族在浆细胞的基因库中占主导地位，这与cDNA克隆的数据一致。用半抗原-载体抗原免疫未诱导全身性抗体反应，并且在组织内浆细胞的聚集或分布模式上也未观察到任何变化。少数浆细胞簇仅表达罕见的VH-I和VH-II家族，表明这些区域正在进行独立于免疫的克隆扩增和分化。
