Artemenko V S, Nenashev V P
Zh Mikrobiol Epidemiol Immunobiol. 1975 Feb(2):85-9.
One of the stages of a new technological scheme for obtaining C1. oedematiens toxoid (detoxication with formaldehyde of a purified concentrated toxin) was elaborated under experimental conditions. It was found that under the effect of increased formalin concentrations (0.4--0.2%) or of prolonged action of heat there developed in the toxin a reduction of antigenic activity, expressed on immunoelectrophoregrams in the form of a change of surface potential of one of the electrophoretic fractions. Detoxication of the purified concentrated C1. oedematients toxin occurred in the course of 3 days without any losses of its antigenic activity under the following conditions: formalin concentration 0.1%, temperature 37-38 degrees C, pH L.7--6.9. No reversion of toxicity of the preparations detoxified under the mentioned conditions was revealed under the action of formalin-binding agent (sodium sulfite) on them.
获得水肿杆菌类毒素（用甲醛对纯化浓缩毒素进行解毒）新技术方案的其中一个阶段是在实验条件下精心制定的。结果发现，在福尔马林浓度增加（0.4% - 0.2%）或加热时间延长的作用下，毒素的抗原活性会降低，在免疫电泳图上表现为其中一个电泳组分的表面电位发生变化。纯化浓缩的水肿杆菌毒素在以下条件下解毒3天，其抗原活性没有任何损失：福尔马林浓度0.1%，温度37 - 38摄氏度，pH值6.7 - 6.9。在上述条件下解毒的制剂在甲醛结合剂（亚硫酸钠）作用下未显示出毒性逆转。
