Takahashi N, Murakami H, Kodama K, Kasagi F, Yamada M, Nishishita T, Inagami T
Department of Genetics, Radiation Effects Research Foundation, 5-2 Hijiyama Park, Minami-ku, Hiroshima, 732-0815, Japan.
Ann Hum Genet. 2000 May;64(Pt 3):197-205. doi: 10.1017/S0003480000008083.
Molecular variants of individual components of the renin-angiotensin system are thought to contribute to inherited predisposition towards essential hypertension. Using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and sequence analysis, we identified seven polymorphisms in the 5'-flanking region of the angiotensin II type 1 receptor (AGTR1/AT 1 ) gene. We conducted a case-control study in a sample from the Japanese population to determine whether polymorphic markers in the 5'-flanking region of the AT 1 gene were associated with essential hypertension. The study compared 149 hypertensive subjects to 156 normotensive control subjects. A significantly higher frequency of the AT 1 (-535)*T allele was observed in hypertensive subjects. Evidence was obtained that the AT 1 (-535)T allele showed a synergistic effect on risk of hypertension with angiotensin I converting enzyme D allele (ACED).
